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@INPROCEEDINGS{Mangiapia:278924,
author = {Mangiapia, Gaetano and D'Errico, Gerardino and
Frielinghaus, Henrich and Lanzetta, Rosa and Molinaro,
Antonio and Paduano, Luigi and Pipich, Vitaliy and
Radulescu, Aurel},
title = {{C}haracterization of liposomes formed by
lipopolysaccharides and their interaction with hybrid
antimicrobial peptides},
reportid = {FZJ-2015-07099},
year = {2015},
abstract = {Lipopolysaccharides (LPSs) are amphiphilic macromolecules
indispensable for the growth and the survival of
Gram-negative bacteria, one of the most diffuse classes of
pathogenic bacteria. LPS are composed of a hydrophilic
heteropolysaccharide unit, covalently linked to a lipophilic
moiety called lipid A, which is embedded in the outer
leaflet and anchors these macromolecules to the lipid
membrane. Recent studies have revealed that presumably the
physical characteristics of these molecules are correlated
to their biological activity.Here we present an
investigation with a twofold goal. First, we try to connect
LPS aggregation behavior to their molecular structure.
Second, we attempt to investigate on a possible interaction
mechanism between LPS model membranes and hybrid
antimicrobial peptides used in the cystic fibrosis therapy
treatment.All the investigations have been performed using
an experimental strategy which has been proved to be
extremely informative, combining dynamic light scattering
(DLS) to estimate liposome dimension, small angle neutron
scattering (SANS) to analyze the aggregate morphology and
for estimating the thickness of the lipid bilayer and
electron paramagnetic resonance spectroscopy (EPR) to
investigate the dynamics of the lipids in the bilayer. These
techniques have also been useful to give insights on the
effect of antimicrobial peptides on the lipid bilayer.},
month = {Nov},
date = {2015-11-10},
organization = {Jülich Soft Matter Days 2015, Bad
Honnef (Germany), 10 Nov 2015 - 13 Nov
2015},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6215 / G:(DE-HGF)POF3-6G15 /
G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)KWS2-20140101 / EXP:(DE-MLZ)KWS3-20140101},
typ = {PUB:(DE-HGF)24},
url = {https://juser.fz-juelich.de/record/278924},
}