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@ARTICLE{Bratanov:279386,
      author       = {Bratanov, Dmitry and Balandin, Taras and Round, Ekaterina
                      and Shevchenko, Vitaly and Gushchin, Ivan and Polovinkin,
                      Vitaly and Borshchevskiy, Valentin and Gordeliy, Valentin},
      title        = {{A}n {A}pproach to {H}eterologous {E}xpression of
                      {M}embrane {P}roteins. {T}he {C}ase of {B}acteriorhodopsin},
      journal      = {PLoS one},
      volume       = {10},
      number       = {6},
      issn         = {1932-6203},
      address      = {Lawrence, Kan.},
      publisher    = {PLoS},
      reportid     = {FZJ-2015-07398},
      pages        = {e0128390 -},
      year         = {2015},
      abstract     = {Heterologous overexpression of functional membrane proteins
                      is a major bottleneck of structural biology.
                      Bacteriorhodopsin from Halobium salinarum (bR) is a striking
                      example of the difficulties in membrane protein
                      overexpression. We suggest a general approach with a finite
                      number of steps which allows one to localize the underlying
                      problem of poor expression of a membrane protein using bR as
                      an example. Our approach is based on constructing chimeric
                      proteins comprising parts of a protein of interest and
                      complementary parts of a homologous protein demonstrating
                      advantageous expression. This complementary protein approach
                      allowed us to increase bR expression by two orders of
                      magnitude through the introduction of two silent mutations
                      into bR coding DNA. For the first time the high quality
                      crystals of bR expressed in E. Coli were obtained using the
                      produced protein. The crystals obtained with in meso
                      nanovolume crystallization diffracted to 1.67 Å.},
      cin          = {ICS-6},
      ddc          = {500},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000355652200066},
      pubmed       = {pmid:26046789},
      doi          = {10.1371/journal.pone.0128390},
      url          = {https://juser.fz-juelich.de/record/279386},
}