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@ARTICLE{Ratanabanangkoon:28022,
      author       = {Ratanabanangkoon, P. and Gropper, M. and Merkel, R. and
                      Sackmann, M. J. and Gast, A. P.},
      title        = {{M}echanics of streptavidin-coated giant lipid bilayer
                      vesicles : a micropipet study},
      journal      = {Langmuir},
      volume       = {19},
      issn         = {0743-7463},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {PreJuSER-28022},
      pages        = {1054 - 1062},
      year         = {2003},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {To understand the effects of a crystalline protein layer on
                      bilayer properties, we studied the mechanical properties of
                      avidin- and streptavidin-coated giant lipid bilayer
                      vesicles. The giant vesicles (20-60 mum) are made from a
                      mixture of SOPC and biotinylated phospholipids via
                      electroformation. Using micropipet manipulation, we showed
                      that the presence of a monomolecular layer of noncrystalline
                      avidin on the vesicle surface increases the membrane bending
                      rigidity but does not significantly alter the elastic area
                      expansion modulus of the vesicle. When the vesicles were
                      coated with streptavidin, the protein crystallizes on the
                      bilayer surface, resulting in a rigid polycrystalline
                      membrane. These vesicles display unique roughened spherical
                      or prolate ellipsoidal shapes, depending on the differences
                      in crystal morphologies. Upon aspiration with micropipets,
                      the vesicles first showed rapid permanent deformation at low
                      strain, followed by a slower viscoelastic response above a
                      certain threshold. Despite their extremely rigid appearance,
                      the existence of a polycrystalline shell does not increase
                      the toughness of streptavidin-coated vesicles above that of
                      uncoated vesicles. The origin of these properties can be
                      traced to the unique ligand-receptor interactions between
                      streptavidin and biotinylated phospholipids in the bilayer
                      membrane. The findings offer greater understandings of
                      complex phenomena involving crystalline protein layers on
                      the surface of cell membranes, in addition to providing
                      information for the development of various applications
                      involving the immobilization of functionalized molecules on
                      lipid bilayer substrates.},
      keywords     = {J (WoSType)},
      cin          = {ISG-4},
      ddc          = {670},
      cid          = {I:(DE-Juel1)VDB44},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK242},
      shelfmark    = {Chemistry, Multidisciplinary / Chemistry, Physical /
                      Materials Science, Multidisciplinary},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000181017700012},
      doi          = {10.1021/la026251y},
      url          = {https://juser.fz-juelich.de/record/28022},
}