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@ARTICLE{GaikoShcherbak:280377,
      author       = {Gaiko-Shcherbak, Aljona and Fabris, Gloria and Dreissen,
                      Georg and Merkel, Rudolf and Hoffmann, Bernd and Noetzel,
                      Erik},
      title        = {{T}he {A}cinar {C}age: {B}asement {M}embranes {D}etermine
                      {M}olecule {E}xchange and {M}echanical {S}tability of
                      {H}uman {B}reast {C}ell {A}cini},
      journal      = {PLoS one},
      volume       = {10},
      number       = {12},
      issn         = {1932-6203},
      address      = {Lawrence, Kan.},
      publisher    = {PLoS},
      reportid     = {FZJ-2016-00154},
      pages        = {e0145174 -},
      year         = {2015},
      abstract     = {The biophysical properties of the basement membrane that
                      surrounds human breast glands are poorly understood, but are
                      thought to be decisive for normal organ function and
                      malignancy. Here, we characterize the breast gland basement
                      membrane with a focus on molecule permeation and mechanical
                      stability, both crucial for organ function. We used
                      well-established and nature-mimicking MCF10A acini as 3D
                      cell model for human breast glands, with ether low- or
                      highly-developed basement membrane scaffolds.
                      Semi-quantitative dextran tracer (3 to 40 kDa) experiments
                      allowed us to investigate the basement membrane scaffold as
                      a molecule diffusion barrier in human breast acini in vitro.
                      We demonstrated that molecule permeation correlated
                      positively with macromolecule size and intriguingly also
                      with basement membrane development state, revealing a pore
                      size of at least 9 nm. Notably, an intact collagen IV mesh
                      proved to be essential for this permeation function.
                      Furthermore, we performed ultra-sensitive atomic force
                      microscopy to quantify the response of native breast acini
                      and of decellularized basement membrane shells against
                      mechanical indentation. We found a clear correlation between
                      increasing acinar force resistance and basement membrane
                      formation stage. Most important native acini with
                      highly-developed basement membranes as well as cell-free
                      basement membrane shells could both withstand
                      physiologically relevant loads (≤ 20 nN) without loss of
                      structural integrity. In contrast, low-developed basement
                      membranes were significantly softer and more fragile. In
                      conclusion, our study emphasizes the key role of the
                      basement membrane as conductor of acinar molecule influx and
                      mechanical stability of human breast glands, which are
                      fundamental for normal organ function.},
      cin          = {ICS-7},
      ddc          = {500},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000366722700110},
      doi          = {10.1371/journal.pone.0145174},
      url          = {https://juser.fz-juelich.de/record/280377},
}