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@ARTICLE{Li:280466,
author = {Li, Jinyu and Santambrogio, Carlo and Brocca, Stefania and
Rossetti, Giulia and Carloni, Paolo and Grandori, Rita},
title = {{C}onformational effects in protein electrospray-ionization
mass spectrometry},
journal = {Mass spectrometry reviews},
volume = {35},
number = {1},
issn = {0277-7037},
address = {New York, NY [u.a.]},
publisher = {Wiley},
reportid = {FZJ-2016-00240},
pages = {111 - 122},
year = {2016},
abstract = {Electrospray-ionization mass spectrometry (ESI-MS) is a key
tool of structural biology, complementing the information
delivered by conventional biochemical and biophysical
methods. Yet, the mechanism behind the conformational
effects in protein ESI-MS is an object of debate. Two
parameters—solvent-accessible surface area (As) and
apparent gas-phase basicity (GBapp)—are thought to play a
role in controlling the extent of protein ionization during
ESI-MS experiments. This review focuses on recent
experimental and theoretical investigations concerning the
influence of these parameters on ESI-MS results and the
structural information that can be derived. The available
evidence supports a unified model for the ionization
mechanism of folded and unfolded proteins. These data
indicate that charge-state distribution (CSD) analysis can
provide valuable structural information on normally folded,
as well as disordered structures.},
cin = {JSC / INM-9 / IAS-5 / GRS Jülich ; German Research School
for Simulation Sciences},
ddc = {530},
cid = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)INM-9-20140121 /
I:(DE-Juel1)IAS-5-20120330 / I:(DE-Juel1)GRS-20100316},
pnm = {511 - Computational Science and Mathematical Methods
(POF3-511) / 574 - Theory, modelling and simulation
(POF3-574)},
pid = {G:(DE-HGF)POF3-511 / G:(DE-HGF)POF3-574},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000368739100007},
pubmed = {pmid:25952139},
doi = {10.1002/mas.21465},
url = {https://juser.fz-juelich.de/record/280466},
}