TY  - JOUR
AU  - Li, Jinyu
AU  - Flick, Franziska
AU  - Verheugd, Patricia
AU  - Carloni, Paolo
AU  - Lüscher, Bernhard
AU  - Rossetti, Giulia
TI  - Insight into the Mechanism of Intramolecular Inhibition of the Catalytic Activity of Sirtuin 2 (SIRT2)
JO  - PLoS one
VL  - 10
IS  - 9
SN  - 1932-6203
CY  - Lawrence, Kan.
PB  - PLoS
M1  - FZJ-2016-00242
SP  - e0139095 -
PY  - 2015
AB  - Sirtuin 2 (SIRT2) is a NAD+-dependent deacetylase that has been associated with neurodegeneration and cancer. SIRT2 is composed of a central catalytic domain, the structure of which has been solved, and N- and C-terminal extensions that are thought to control SIRT2 function. However structural information of these N- and C-terminal regions is missing. Here, we provide the first full-length molecular models of SIRT2 in the absence and presence of NAD+. We also predict the structural alterations associated with phosphorylation of SIRT2 at S331, a modification that inhibits catalytic activity. Bioinformatics tools and molecular dynamics simulations, complemented by in vitro deacetylation assays, provide a consistent picture based on which the C-terminal region of SIRT2 is suggested to function as an autoinhibitory region. This has the capacity to partially occlude the NAD+ binding pocket or stabilize the NAD+ in a non-productive state. Furthermore, our simulations suggest that the phosphorylation at S331 causes large conformational changes in the C-terminal region that enhance the autoinhibitory activity, consistent with our previous findings that phosphorylation of S331 by cyclin-dependent kinases inhibits SIRT2 catalytic activity. The molecular insight into the role of the C-terminal region in controlling SIRT2 function described in this study may be useful for future design of selective inhibitors targeting SIRT2 for therapeutic applications.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000361800700166
C6  - pmid:26407304
DO  - DOI:10.1371/journal.pone.0139095
UR  - https://juser.fz-juelich.de/record/280468
ER  -