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@ARTICLE{Domrse:280734,
      author       = {Domröse, Andreas and Klein, A. S. and Hage-Hülsmann,
                      Jennifer and Thies, Stephan and Svensson, V. and Classen,
                      Thomas and Pietruszka, Jörg and Jaeger, Karl-Erich and
                      Drepper, Thomas and Loeschcke, Anita},
      title        = {{E}fficient recombinant production of prodigiosin in
                      {P}seudomonas putida},
      journal      = {Frontiers in microbiology},
      volume       = {6},
      issn         = {1664-302X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2016-00491},
      pages        = {972},
      year         = {2015},
      abstract     = {Serratia marcescens and several other bacteria produce the
                      red-colored pigment prodigiosin which possesses
                      bioactivities as an antimicrobial, anticancer, and
                      immunosuppressive agent. Therefore, there is a great
                      interest to produce this natural compound. Efforts aiming at
                      its biotechnological production have so far largely focused
                      on the original producer and opportunistic human pathogen S.
                      marcescens. Here, we demonstrate efficient prodigiosin
                      production in the heterologous host Pseudomonas putida.
                      Random chromosomal integration of the 21 kb prodigiosin
                      biosynthesis gene cluster of S. marcescens in P. putida
                      KT2440 was employed to construct constitutive prodigiosin
                      production strains. Standard cultivation parameters were
                      optimized such that titers of 94 mg/L culture were obtained
                      upon growth of P. putida at 20∘C using rich medium under
                      high aeration conditions. Subsequently, a novel, fast and
                      effective protocol for prodigiosin extraction and
                      purification was established enabling the straightforward
                      isolation of prodigiosin from P. putida growth medium. In
                      summary, we describe here a highly efficient method for the
                      heterologous biosynthetic production of prodigiosin which
                      may serve as a basis to produce large amounts of this
                      bioactive natural compound and may provide a platform for
                      further in-depth studies of prodiginine biosynthesis.},
      cin          = {IMET / IBOC / IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IMET-20090612 / I:(DE-Juel1)IBOC-20090406 /
                      I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000361381600001},
      pubmed       = {pmid:26441905},
      doi          = {10.3389/fmicb.2015.00972},
      url          = {https://juser.fz-juelich.de/record/280734},
}