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@ARTICLE{Belu:281937,
      author       = {Belu, Andreea and Schnitker, Jan and Bertazzo, S. and
                      Neumann, Elmar and Mayer, Dirk and Offenhäusser, Andreas
                      and Santoro, Francesca},
      title        = {{U}ltra-thin resin embedding method for scanning electron
                      microscopy of individual cells on high and low aspect ratio
                      3{D} nanostructures},
      journal      = {Journal of microscopy},
      volume       = {263},
      number       = {1},
      issn         = {0022-2720},
      address      = {Oxford [u.a.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2016-01587},
      pages        = {78–86},
      year         = {2016},
      abstract     = {The preparation of biological cells for either scanning or
                      transmission electron microscopy requires a complex process
                      of fixation, dehydration and drying. Critical point drying
                      is commonly used for samples investigated with a scanning
                      electron beam, whereas resin-infiltration is typically used
                      for transmission electron microscopy. Critical point drying
                      may cause cracks at the cellular surface and a sponge-like
                      morphology of nondistinguishable intracellular compartments.
                      Resin-infiltrated biological samples result in a solid block
                      of resin, which can be further processed by mechanical
                      sectioning, however that does not allow a top view
                      examination of small cell–cell and cell–surface
                      contacts. Here, we propose a method for removing resin
                      excess on biological samples before effective
                      polymerization. In this way the cells result to be embedded
                      in an ultra-thin layer of epoxy resin. This novel method
                      highlights in contrast to standard methods the imaging of
                      individual cells not only on nanostructured planar surfaces
                      but also on topologically challenging substrates with high
                      aspect ratio three-dimensional features by scanning electron
                      microscopy.},
      cin          = {ICS-8 / PGI-8-PT},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-8-20110106 / I:(DE-Juel1)PGI-8-PT-20110228},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000379167100008},
      pubmed       = {pmid:26820619},
      doi          = {10.1111/jmi.12378},
      url          = {https://juser.fz-juelich.de/record/281937},
}