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000002833 0247_ $$2DOI$$a10.1104/pp.108.134486
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000002833 041__ $$aeng
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000002833 084__ $$2WoS$$aPlant Sciences
000002833 1001_ $$0P:(DE-Juel1)VDB62682$$aBiskup, B.$$b0$$uFZJ
000002833 245__ $$aDiel growth cycle of isolated leaf discs analyzed with a novel, high-throughput three-dimensional imaging method is identical to that of intact leaves
000002833 260__ $$aRockville, Md.: Soc.$$bJSTOR$$c2009
000002833 300__ $$a1452 - 1461
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000002833 3367_ $$2DRIVER$$aarticle
000002833 440_0 $$04987$$aPlant Physiology$$v149$$x0032-0889
000002833 500__ $$aThis work was supported by Forschungszentrum Julich GmbH. B. B. acknowledges support of his Ph. D. thesis by the Heinrich-Heine University of Dusseldorf, Germany.
000002833 520__ $$aDicot leaves grow with pronounced diel (24-h) cycles that are controlled by a complex network of factors. It is an open question to what extent leaf growth dynamics are controlled by long-range or by local signals. To address this question, we established a stereoscopic imaging system, GROWSCREEN 3D, which quantifies surface growth of isolated leaf discs floating on nutrient solution in wells of microtiter plates. A total of 458 leaf discs of tobacco (Nicotiana tabacum) were cut at different developmental stages, incubated, and analyzed for their relative growth rates. The camera system was automatically displaced across the array of leaf discs; visualization and camera displacement took about 12 s for each leaf disc, resulting in a time interval of 1.5 h for consecutive size analyses. Leaf discs showed a comparable diel leaf growth cycle as intact leaves but weaker peak growth activity. Hence, it can be concluded that the timing of leaf growth is regulated by local rather than by systemic control processes. This conclusion was supported by results from leaf discs of Arabidopsis (Arabidopsis thaliana) Landsberg erecta wild-type plants and starch-free1 mutants. At night, utilization of transitory starch leads to increased growth of Landsberg erecta wild-type discs compared with starch-free1 discs. Moreover, the decrease of leaf disc growth when exposed to different concentrations of glyphosate showed an immediate dose-dependent response. Our results demonstrate that a dynamic leaf disc growth analysis as we present it here is a promising approach to uncover the effects of internal and external cues on dicot leaf development.
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000002833 588__ $$aDataset connected to Web of Science, Pubmed
000002833 650_2 $$2MeSH$$aArabidopsis: genetics
000002833 650_2 $$2MeSH$$aArabidopsis: growth & development
000002833 650_2 $$2MeSH$$aCircadian Rhythm
000002833 650_2 $$2MeSH$$aGenotype
000002833 650_2 $$2MeSH$$aImaging, Three-Dimensional: methods
000002833 650_2 $$2MeSH$$aPlant Leaves: growth & development
000002833 650_2 $$2MeSH$$aSolutions
000002833 650_2 $$2MeSH$$aTime Factors
000002833 650_2 $$2MeSH$$aTobacco: growth & development
000002833 650_7 $$00$$2NLM Chemicals$$aSolutions
000002833 650_7 $$2WoSType$$aJ
000002833 7001_ $$0P:(DE-Juel1)129394$$aScharr, H.$$b1$$uFZJ
000002833 7001_ $$0P:(DE-Juel1)129315$$aFischbach, A.$$b2$$uFZJ
000002833 7001_ $$0P:(DE-Juel1)129420$$aWiese-Klinkenberg, A.$$b3$$uFZJ
000002833 7001_ $$0P:(DE-Juel1)129402$$aSchurr, U.$$b4$$uFZJ
000002833 7001_ $$0P:(DE-Juel1)VDB2595$$aWalter, A.$$b5$$uFZJ
000002833 773__ $$0PERI:(DE-600)2004346-6$$a10.1104/pp.108.134486$$gVol. 149, p. 1452 - 1461$$p1452 - 1461$$q149<1452 - 1461$$tPlant physiology$$v149$$x0032-0889$$y2009
000002833 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649408
000002833 8564_ $$uhttps://juser.fz-juelich.de/record/2833/files/FZJ-2833.pdf$$yRestricted$$zPublished final document.
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