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@ARTICLE{Biskup:2833,
      author       = {Biskup, B. and Scharr, H. and Fischbach, A. and
                      Wiese-Klinkenberg, A. and Schurr, U. and Walter, A.},
      title        = {{D}iel growth cycle of isolated leaf discs analyzed with a
                      novel, high-throughput three-dimensional imaging method is
                      identical to that of intact leaves},
      journal      = {Plant physiology},
      volume       = {149},
      issn         = {0032-0889},
      address      = {Rockville, Md.: Soc.},
      publisher    = {JSTOR},
      reportid     = {PreJuSER-2833},
      pages        = {1452 - 1461},
      year         = {2009},
      note         = {This work was supported by Forschungszentrum Julich GmbH.
                      B. B. acknowledges support of his Ph. D. thesis by the
                      Heinrich-Heine University of Dusseldorf, Germany.},
      abstract     = {Dicot leaves grow with pronounced diel (24-h) cycles that
                      are controlled by a complex network of factors. It is an
                      open question to what extent leaf growth dynamics are
                      controlled by long-range or by local signals. To address
                      this question, we established a stereoscopic imaging system,
                      GROWSCREEN 3D, which quantifies surface growth of isolated
                      leaf discs floating on nutrient solution in wells of
                      microtiter plates. A total of 458 leaf discs of tobacco
                      (Nicotiana tabacum) were cut at different developmental
                      stages, incubated, and analyzed for their relative growth
                      rates. The camera system was automatically displaced across
                      the array of leaf discs; visualization and camera
                      displacement took about 12 s for each leaf disc, resulting
                      in a time interval of 1.5 h for consecutive size analyses.
                      Leaf discs showed a comparable diel leaf growth cycle as
                      intact leaves but weaker peak growth activity. Hence, it can
                      be concluded that the timing of leaf growth is regulated by
                      local rather than by systemic control processes. This
                      conclusion was supported by results from leaf discs of
                      Arabidopsis (Arabidopsis thaliana) Landsberg erecta
                      wild-type plants and starch-free1 mutants. At night,
                      utilization of transitory starch leads to increased growth
                      of Landsberg erecta wild-type discs compared with
                      starch-free1 discs. Moreover, the decrease of leaf disc
                      growth when exposed to different concentrations of
                      glyphosate showed an immediate dose-dependent response. Our
                      results demonstrate that a dynamic leaf disc growth analysis
                      as we present it here is a promising approach to uncover the
                      effects of internal and external cues on dicot leaf
                      development.},
      keywords     = {Arabidopsis: genetics / Arabidopsis: growth $\&$
                      development / Circadian Rhythm / Genotype / Imaging,
                      Three-Dimensional: methods / Plant Leaves: growth $\&$
                      development / Solutions / Time Factors / Tobacco: growth
                      $\&$ development / Solutions (NLM Chemicals) / J (WoSType)},
      cin          = {ICG-3},
      ddc          = {580},
      cid          = {I:(DE-Juel1)ICG-3-20090406},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Plant Sciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19168641},
      pmc          = {pmc:PMC2649408},
      UT           = {WOS:000263843700020},
      doi          = {10.1104/pp.108.134486},
      url          = {https://juser.fz-juelich.de/record/2833},
}