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| Home > Publications database > Development of a high-throughput agar-based root and shoot phenotyping system |
| Conference Presentation (After Call) | FZJ-2016-01772 |
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2016
Abstract: Roots are most of the time not directly visible, but are of outstanding importance for plant nutrition. As we are using plants for our nutrition and other economic needs there are as well of big importance for everybody. Nevertheless plant research has not been driven very much by roots as this always implies the problem of monitoring, measuring and evaluating a well-hidden belowground structure which has to be made accessible for these tasks. Gathering a deeper knowledge about root structure, development and behavior is important because this will influence our possibilities of improving plant productivity and usability under stress conditions (e.g. limited nutrients, water, salt) in the future.For this reason several screening systems have been developed in recent years which are trying to overcome the problem of visibility and throughput to cope with the need for screening huge plant collections either for getting basic knowledge or for finding root traits useable in plant breeding. One of these systems is an agar based 2D system where seedlings or smaller plants can be grown in transparent media with defined composition while being able to monitor whole root development inside and shoot development outside of the container at the same time. In the present system the plants are grown in commercially available Petri dishes which have to be modified to allow the shoot to grow outside the plate. Individual plate preparation is quite time consuming and therefore not suitable for high throughput phenotyping. Consequently, one goal for the high throughput installation, which is currently developed within the DPPN project, is a reduction of manual production. To solve this we are currently testing special designed agar-filled plates, in parallel, various transport and imaging concepts are evaluated. The current concept is a modular design in which the agar-filled plates will be grouped in trays, in which they will be transported to the imaging station for shoot and root measurements. This concept allows also randomization and will enable screening of thousands of plants without manual interaction. Furthermore, the installation will also be useable for phenotyping plants grown in soil filled pots which makes it flexible for different types of experimental questions.
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