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@ARTICLE{Braun:283561,
author = {Braun, Ramona and Klein, Rebecca and Walter, Helene Luise
and Ohren, Maurice and Freudenmacher, Lars and Getachew,
Kaleab and Ladwig, Anne and Luelling, Joachim and Neumaier,
Bernd and Endepols, Heike and Graf, Rudolf and Hoehn,
Mathias and Fink, Gereon Rudolf and Schroeter, Michael and
Rueger, Maria Adele},
title = {{T}ranscranial direct current stimulation accelerates
recovery of function, induces neurogenesis and recruits
oligodendrocyte precursors in a rat model of stroke},
journal = {Experimental neurology},
volume = {279},
issn = {0014-4886},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {FZJ-2016-01876},
pages = {127 - 136},
year = {2016},
abstract = {BackgroundClinical data suggest that transcranial direct
current stimulation (tDCS) may be used to facilitate
rehabilitation after stroke. However, data are inconsistent
and the neurobiological mechanisms underlying tDCS remain
poorly explored, impeding its implementation into clinical
routine. In the healthy rat brain, tDCS affects neural stem
cells (NSC) and microglia. We here investigated whether tDCS
applied after stroke also beneficially affects these cells,
which are known to be involved in regeneration and
repair.MethodsFocal cerebral ischemia was induced in rats by
transient occlusion of the middle cerebral artery.
Twenty-eight animals with comparable infarcts, as judged by
magnetic resonance imaging, were randomized to receive a
multi-session paradigm of either cathodal, anodal, or sham
tDCS. Behaviorally, recovery of motor function was assessed
by Catwalk. Proliferation in the NSC niches was monitored by
Positron-Emission-Tomography (PET) employing the radiotracer
3′-deoxy-3′-[18F]fluoro-l-thymidine ([18F]FLT).
Microglia activation was depicted with [11C]PK11195-PET. In
addition, immunohistochemical analyses were used to quantify
neuroblasts, oligodendrocyte precursors, and activation and
polarization of microglia.ResultsAnodal and cathodal tDCS
both accelerated functional recovery, though affecting
different aspects of motor function. Likewise, tDCS induced
neurogenesis independently of polarity, while only cathodal
tDCS recruited oligodendrocyte precursors towards the
lesion. Moreover, cathodal stimulation preferably supported
M1-polarization of microglia.ConclusionsTDCS acts through
multifaceted mechanisms that far exceed its primary
neurophysiological effects, encompassing proliferation and
migration of stem cells, their neuronal differentiation, and
modulation of microglia responses.},
cin = {INM-3 / INM-5},
ddc = {610},
cid = {I:(DE-Juel1)INM-3-20090406 / I:(DE-Juel1)INM-5-20090406},
pnm = {572 - (Dys-)function and Plasticity (POF3-572)},
pid = {G:(DE-HGF)POF3-572},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000374612900011},
pubmed = {pmid:26923911},
doi = {10.1016/j.expneurol.2016.02.018},
url = {https://juser.fz-juelich.de/record/283561},
}