TY  - JOUR
AU  - Kaupp, U. B.
AU  - Solzin, J.
AU  - Hildebrand, E.
AU  - Brown, J. E.
AU  - Helbig, A.
AU  - Hagen, V.
AU  - Beyermann, M.
AU  - Pampaloni, F.
AU  - Weyand, I.
TI  - The signal flow and motor response controling chemotaxis of sea urchin sperm
JO  - Nature cell biology
VL  - 5
SN  - 1465-7392
CY  - New York, NY
PB  - Nature America
M1  - PreJuSER-28485
SP  - 109 - 117
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - The signalling pathway and the behavioural strategy underlying chemotaxis of sperm are poorly understood. We have studied the cellular events and motor responses that mediate chemotaxis of sperm from the sea urchin Arbacia punctulata. Here we show that resact, a chemoattractant peptide, initiates a rapid and transient rise in the concentration of cyclic GMP, followed by a transient influx of Ca2+. The binding of a single resact molecule elicits a Ca2+ response, and 50-100 bound molecules saturate the response. The ability to register single molecules is reminiscent of the single-photon sensitivity of rod photoreceptors. Both resact and cyclic nucleotides cause a turn or brief tumbling in the swimming path of sperm. We conclude that a cGMP-mediated increase in the Ca2+ concentration induces the primary motor response of sperm to the chemoattractant.
KW  - Animals
KW  - Calcium: metabolism
KW  - Chemotaxis: physiology
KW  - Cyclic AMP: analogs & derivatives
KW  - Cyclic AMP: metabolism
KW  - Cyclic GMP: analogs & derivatives
KW  - Cyclic GMP: metabolism
KW  - Egg Proteins: metabolism
KW  - Guanylate Cyclase
KW  - Male
KW  - Molecular Structure
KW  - Protein Binding
KW  - Receptors, Cell Surface: metabolism
KW  - Sea Urchins: physiology
KW  - Signal Transduction: physiology
KW  - Sperm Motility
KW  - Spermatozoa: cytology
KW  - Spermatozoa: metabolism
KW  - Spermatozoa: physiology
KW  - Egg Proteins (NLM Chemicals)
KW  - Receptors, Cell Surface (NLM Chemicals)
KW  - Cyclic AMP (NLM Chemicals)
KW  - Calcium (NLM Chemicals)
KW  - Cyclic GMP (NLM Chemicals)
KW  - Guanylate Cyclase (NLM Chemicals)
KW  - resact protein, sea urchin (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:12563276
UR  - <Go to ISI:>//WOS:000180802100010
DO  - DOI:10.1038/ncb915
UR  - https://juser.fz-juelich.de/record/28485
ER  -