TY  - JOUR
AU  - Wiesehan, K.
AU  - Buder, K.
AU  - Linke, R. P.
AU  - Patt, S.
AU  - Stoldt, M.
AU  - Unger, E.
AU  - Schmitt, B.
AU  - Bucci, E.
AU  - Willbold, D.
TI  - Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide A beta(1-42) by mirror image phage display
JO  - ChemBioChem
VL  - 4
SN  - 1439-4227
CY  - Weinheim
PB  - Wiley-VCH
M1  - PreJuSER-29871
SP  - 748 - 753
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide Abeta(1-42). A randomized 12-mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of Abeta(1-42). After four rounds of selection and amplification the peptides were enriched with a dominating consensus sequence. The mirror image of the most representative peptide (D-pep) was shown to bind Abeta(1-42) with a dissociation constant in the submicromolar range. Furthermore, in brain tissue sections derived from patients that suffered from Alzheimer's disease, amyloid plaques and leptomeningeal vessels containing Abeta amyloid were stained specifically with a fluorescence-labeled derivative of D-pep. Fibrillar deposits derived from other amyloidosis were not labeled by D-pep. Possible applications of this novel and highly specific Abeta ligand in diagnosis and therapy of Alzheimer's disease are discussed.
KW  - Alzheimer Disease: metabolism
KW  - Amino Acid Sequence
KW  - Amyloid beta-Peptides: chemistry
KW  - Amyloid beta-Peptides: genetics
KW  - Amyloid beta-Peptides: metabolism
KW  - Amyloidosis: metabolism
KW  - Brain: metabolism
KW  - Humans
KW  - Ligands
KW  - Molecular Sequence Data
KW  - Peptide Fragments: chemistry
KW  - Peptide Fragments: genetics
KW  - Peptide Fragments: metabolism
KW  - Peptide Library
KW  - Plaque, Amyloid: genetics
KW  - Plaque, Amyloid: metabolism
KW  - Protein Binding: physiology
KW  - Amyloid beta-Peptides (NLM Chemicals)
KW  - Ligands (NLM Chemicals)
KW  - Peptide Fragments (NLM Chemicals)
KW  - Peptide Library (NLM Chemicals)
KW  - amyloid beta-protein (1-40) (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:12898626
UR  - <Go to ISI:>//WOS:000184660400010
DO  - DOI:10.1002/cbic.200300631
UR  - https://juser.fz-juelich.de/record/29871
ER  -