TY  - JOUR
AU  - Preußer, A.
AU  - Jonas, G.
AU  - Willbold, D.
TI  - Purification of recombinantly expressed human cluster determinant 4 cytoplasmatic domain
JO  - Journal of chromatography / B
VL  - 786
SN  - 1570-0232
CY  - New York, NY [u.a.]
PB  - Science Direct
M1  - PreJuSER-30237
SP  - 39 - 44
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - A DNA fragment coding for the human CD4 cytoplasmic domain (residues 394-433) was cloned into the pET15b expression vector. The resulting plasmid was used for synthesis of the polyhistidine-tagged 5.10(3) M-r CD4 peptide in Escherichia coli BL21(DE3)Star. The CD4 cytoplasmic domain was purified under denaturing and reducing conditions by a two-step procedure using immobilized metal affinity chromatography and gel permeation chromatography. The purified CD4 cytoplasmic domain is soluble and functional without any specific refolding steps. The yield of the described purification procedure was similar to5 mg peptide per liter culture volume. (C) 2002 Elsevier Science B.V. All rights reserved.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000181406500006
DO  - DOI:10.1016/S1570-0232(02)00731-6
UR  - https://juser.fz-juelich.de/record/30237
ER  -