% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Preuer:30237,
      author       = {Preußer, A. and Jonas, G. and Willbold, D.},
      title        = {{P}urification of recombinantly expressed human cluster
                      determinant 4 cytoplasmatic domain},
      journal      = {Journal of chromatography / B},
      volume       = {786},
      issn         = {1570-0232},
      address      = {New York, NY [u.a.]},
      publisher    = {Science Direct},
      reportid     = {PreJuSER-30237},
      pages        = {39 - 44},
      year         = {2003},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {A DNA fragment coding for the human CD4 cytoplasmic domain
                      (residues 394-433) was cloned into the pET15b expression
                      vector. The resulting plasmid was used for synthesis of the
                      polyhistidine-tagged 5.10(3) M-r CD4 peptide in Escherichia
                      coli BL21(DE3)Star. The CD4 cytoplasmic domain was purified
                      under denaturing and reducing conditions by a two-step
                      procedure using immobilized metal affinity chromatography
                      and gel permeation chromatography. The purified CD4
                      cytoplasmic domain is soluble and functional without any
                      specific refolding steps. The yield of the described
                      purification procedure was similar to5 mg peptide per liter
                      culture volume. (C) 2002 Elsevier Science B.V. All rights
                      reserved.},
      keywords     = {J (WoSType)},
      cin          = {IBI-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB58},
      pnm          = {Neurowissenschaften},
      pid          = {G:(DE-Juel1)FUEK255},
      shelfmark    = {Biochemical Research Methods / Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000181406500006},
      doi          = {10.1016/S1570-0232(02)00731-6},
      url          = {https://juser.fz-juelich.de/record/30237},
}