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| Home > Publications database > Purification of recombinantly expressed human cluster determinant 4 cytoplasmatic domain > print |
| 001 | 30237 | ||
| 005 | 20200402210517.0 | ||
| 024 | 7 | _ | |2 DOI |a 10.1016/S1570-0232(02)00731-6 |
| 024 | 7 | _ | |2 WOS |a WOS:000181406500006 |
| 037 | _ | _ | |a PreJuSER-30237 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Biochemical Research Methods |
| 084 | _ | _ | |2 WoS |a Chemistry, Analytical |
| 100 | 1 | _ | |a Preußer, A. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a Purification of recombinantly expressed human cluster determinant 4 cytoplasmatic domain |
| 260 | _ | _ | |a New York, NY [u.a.] |b Science Direct |c 2003 |
| 300 | _ | _ | |a 39 - 44 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of Chromatography B |x 1570-0232 |0 3155 |v 786 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a A DNA fragment coding for the human CD4 cytoplasmic domain (residues 394-433) was cloned into the pET15b expression vector. The resulting plasmid was used for synthesis of the polyhistidine-tagged 5.10(3) M-r CD4 peptide in Escherichia coli BL21(DE3)Star. The CD4 cytoplasmic domain was purified under denaturing and reducing conditions by a two-step procedure using immobilized metal affinity chromatography and gel permeation chromatography. The purified CD4 cytoplasmic domain is soluble and functional without any specific refolding steps. The yield of the described purification procedure was similar to5 mg peptide per liter culture volume. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 536 | _ | _ | |a Neurowissenschaften |c L01 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK255 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a purification |
| 653 | 2 | 0 | |2 Author |a Escherichia coli |
| 653 | 2 | 0 | |2 Author |a cluster determinant 4 |
| 700 | 1 | _ | |a Jonas, G. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Willbold, D. |b 2 |u FZJ |0 P:(DE-Juel1)132029 |
| 773 | _ | _ | |a 10.1016/S1570-0232(02)00731-6 |g Vol. 786, p. 39 - 44 |p 39 - 44 |q 786<39 - 44 |0 PERI:(DE-600)1491259-4 |t Journal of chromatography / B |v 786 |y 2003 |x 1570-0232 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1016/S1570-0232(02)00731-6 |
| 909 | C | O | |o oai:juser.fz-juelich.de:30237 |p VDB |
| 913 | 1 | _ | |k L01 |v Neurowissenschaften |l Funktion und Dysfunktion des Nervensystems |b Leben |0 G:(DE-Juel1)FUEK255 |x 0 |
| 914 | 1 | _ | |y 2003 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k IBI-2 |l Biologische Strukturforschung |d 31.12.2006 |g IBI |0 I:(DE-Juel1)VDB58 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)27786 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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