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@ARTICLE{Briese:30238,
author = {Briese, L. and Willbold, D.},
title = {{S}tructure determination of human {L}ck unique and {SH}3
domains by nuclear magnetic resonance spectroscopy},
journal = {BMC structural biology},
volume = {3},
issn = {1472-6807},
address = {London},
publisher = {BioMed Central},
reportid = {PreJuSER-30238},
pages = {3},
year = {2003},
note = {Record converted from VDB: 12.11.2012},
abstract = {Protein tyrosine kinases are involved in signal
transduction pathways that regulate cell growth,
differentiation, activation and transformation. Human
lymphocyte specific kinase (Lck) is a 56 kDa protein
involved in T-cell- and IL2-receptor signaling.
Three-dimensional structures are known for SH3, SH2 and
kinase domains of Lck as well as for other tyrosine kinases.
No structure is known for the unique domain of any Src-type
tyrosine kinase.Lck(1-120) comprising unique and SH3 domains
was structurally investigated by nuclear magnetic resonance
spectroscopy. We found the unique domain, in contrast to the
SH3 part, to have basically no defined structural elements.
The solution structure of the SH3 part could be determined
with very high precision. It does not show significant
differences to Lck SH3 in the absence of the unique domain.
Minor differences were observed to the X-ray structure of
Lck SH3.The unique domain of Lck does not contain any
defined structure elements in the absence of ligands and
membranes. Presence of the unique domain is not relevant to
the three-dimensional structure of the Lck SH3 domain.},
keywords = {Crystallography, X-Ray / Humans / Lymphocyte Specific
Protein Tyrosine Kinase p56(lck): chemistry / Models,
Molecular / Nuclear Magnetic Resonance, Biomolecular / src
Homology Domains / Lymphocyte Specific Protein Tyrosine
Kinase p56(lck) (NLM Chemicals)},
cin = {IBI-2},
ddc = {570},
cid = {I:(DE-Juel1)VDB58},
pnm = {Neurowissenschaften},
pid = {G:(DE-Juel1)FUEK255},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:12734017},
pmc = {pmc:PMC156628},
doi = {10.1186/1472-6807-3-3},
url = {https://juser.fz-juelich.de/record/30238},
}