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| 001 | 30299 | ||
| 005 | 20200402210518.0 | ||
| 024 | 7 | _ | |2 DOI |a 10.1016/S0022-2836(03)00581-3 |
| 024 | 7 | _ | |2 WOS |a WOS:000183824900020 |
| 037 | _ | _ | |a PreJuSER-30299 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 084 | _ | _ | |2 WoS |a Biochemistry & Molecular Biology |
| 100 | 1 | _ | |a Senin, I. I. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a Functional restoration of the Ca2 -myristoyl switch in a recoverin mutant |
| 260 | _ | _ | |a Amsterdam [u.a.] |b Elsevier |c 2003 |
| 300 | _ | _ | |a 409 - 418 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of Molecular Biology |x 0022-2836 |0 3552 |v 330 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Recoverin is a neuronal calcium sensor protein that plays a crucial role in vertebrate phototransduction. It undergoes a Ca2+-myristoyl switch when Ca2+ binds to its two functional EF-hand motifs (EF-hands 2 and 3), each present in one of recoverin's two domains. Impairment of Ca2+-binding in recoverin leads to a disturbance of the Ca2+-myristoyl switch and loss of its regulatory properties, i.e. inhibiton of rhodopsin kinase. We have engineered recoverin mutants with either of the two functional EF-hands disabled, but with a functional Ca2+-binding site in EF-hand 4. While a defect in EF-hand 2 could not be rescued by the additional EF-hand 4, the impairment of EF-hand 3 was powerfully compensated by Ca2+-binding to EF-hand 4. For example, the myristoylated form of the latter mutant bound to membranes in a Ca2+-dependent way and was able to inhibit rhodopsin kinase in a way similar to that of the wild-type protein. Thus, for recoverin to undergo a Ca2+-myristoyl switch, it is necessary and sufficient to have either of the two EF-hands in the second domain in a functional state. On the basis of these results and inspection of published three-dimensional structures of recoverin, we propose a model highlighting the mutual interdependence of sterical configurations in EF-hands 3 and 4 of recoverin. (C) 2003 Elsevier Science Ltd. All rights reserved. |
| 536 | _ | _ | |a Neurowissenschaften |c L01 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK255 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a calcium sensor |
| 653 | 2 | 0 | |2 Author |a EF-hand protein |
| 653 | 2 | 0 | |2 Author |a myristoyl switch |
| 653 | 2 | 0 | |2 Author |a rhodopsin kinase |
| 653 | 2 | 0 | |2 Author |a phototransduction |
| 700 | 1 | _ | |a Vaganova, S. A. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Weiergräber, O. H. |b 2 |u FZJ |0 P:(DE-Juel1)131988 |
| 700 | 1 | _ | |a Ergorov, N. S. |b 3 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Philippov, P. P. |b 4 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Koch, K.-W. |b 5 |u FZJ |0 P:(DE-Juel1)VDB789 |
| 773 | _ | _ | |a 10.1016/S0022-2836(03)00581-3 |g Vol. 330, p. 409 - 418 |p 409 - 418 |q 330<409 - 418 |0 PERI:(DE-600)1355192-9 |t Journal of molecular biology |v 330 |y 2003 |x 0022-2836 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1016/S0022-2836(03)00581-3 |
| 909 | C | O | |o oai:juser.fz-juelich.de:30299 |p VDB |
| 913 | 1 | _ | |k L01 |v Neurowissenschaften |l Funktion und Dysfunktion des Nervensystems |b Leben |0 G:(DE-Juel1)FUEK255 |x 0 |
| 914 | 1 | _ | |y 2003 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k IBI-1 |l Zelluläre Signalverarbeitung |d 31.12.2006 |g IBI |0 I:(DE-Juel1)VDB57 |x 0 |
| 920 | 1 | _ | |k IBI-2 |l Biologische Strukturforschung |d 31.12.2006 |g IBI |0 I:(DE-Juel1)VDB58 |x 1 |
| 970 | _ | _ | |a VDB:(DE-Juel1)28047 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-4-20110106 |
| 980 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-1-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-4-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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