TY  - JOUR
AU  - Uttenweiler, D.
AU  - Weber, C.
AU  - Jähne, B.
AU  - Fink, R. H. A.
AU  - Scharr, H.
TI  - Spatiotemporal anisotropic diffusion filtering to improve  signal-to-noise ratios and object restoration in fluorescence microscopic image sequences
JO  - Journal of biomedical optics
VL  - 8
SN  - 1083-3668
CY  - Bellingham, Wash.
PB  - SPIE
M1  - PreJuSER-30346
SP  - 40 - 47
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - We present an approach for significantly improving the quantitative analysis of motion in noisy fluorescence microscopic image sequences. The new partial differential equation based method is a general extension of a 2-D nonlinear anisotropic diffusion filtering scheme to a specially adapted 3-D nonlinear anisotropic diffusion filtering scheme, with two spatial image dimensions and the time t in the image sequence as the third dimension. Motion in image sequences is considered as oriented, line-like structures in the spatiotemporal x,y,t domain, which are determined by the structure tensor method. Image enhancement is achieved by a structure adopted smoothing kernel in three dimensions, thereby using the full 3-D information inherent in spatiotemporal image sequences. As an example for low signal-to-noise ratio (SNR) microscopic image sequences we have applied this method to noisy in vitro motility assay data, where fluorescently labeled actin filaments move over a surface of immobilized myosin. With the 3-D anisotropic diffusion filtering the SNR is significantly improved (by a factor of 3.8) and closed object structures are reliably restored, which were originally degraded by noise. Generally, this approach is very valuable for all applications where motion has to be measured quantitatively in low light level fluorescence microscopic image sequences of cellular, subcellular, and molecular processes.
KW  - Actins: chemistry
KW  - Actins: ultrastructure
KW  - Animals
KW  - Fluorescence Polarization: methods
KW  - Image Processing, Computer-Assisted
KW  - Microscopy, Fluorescence: methods
KW  - Motion
KW  - Rabbits
KW  - Actins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:12542378
UR  - <Go to ISI:>//WOS:000180755400005
DO  - DOI:10.1117/1.1527627
UR  - https://juser.fz-juelich.de/record/30346
ER  -