000030442 001__ 30442
000030442 005__ 20200402210520.0
000030442 0247_ $$2DOI$$a10.1023/A:1023887412387
000030442 0247_ $$2WOS$$aWOS:000183041200001
000030442 037__ $$aPreJuSER-30442
000030442 041__ $$aeng
000030442 082__ $$a570
000030442 084__ $$2WoS$$aBiochemistry & Molecular Biology
000030442 084__ $$2WoS$$aSpectroscopy
000030442 1001_ $$0P:(DE-Juel1)132009$$aKoenig, B. W.$$b0$$uFZJ
000030442 245__ $$aA rapid method to attain isotope labeled small soluble peptides for NMR studies
000030442 260__ $$aDordrecht [u.a.]$$bSpringer Science + Business Media B.V$$c2003
000030442 300__ $$a193 - 202
000030442 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000030442 3367_ $$2DataCite$$aOutput Types/Journal article
000030442 3367_ $$00$$2EndNote$$aJournal Article
000030442 3367_ $$2BibTeX$$aARTICLE
000030442 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000030442 3367_ $$2DRIVER$$aarticle
000030442 440_0 $$09165$$aJournal of Biomolecular NMR$$v26$$x0925-2738
000030442 500__ $$aRecord converted from VDB: 12.11.2012
000030442 520__ $$aA widely applicable strategy is presented for efficient and rapid production of small water soluble peptides expressed as fusion proteins with the immunoglobulin-binding domain of streptococcal protein G. A simple extraction and purification scheme that includes a protease cleavage step to release the target peptide is described. The yield of authentic target peptide exceeds 10 mg per liter of culture. Production of U-C-13, N-15 and highly deuterated U-C-13, N-15 isotope labeled peptide is demonstrated for the 11 residue S2 peptide, corresponding to the C-terminus of the alpha-subunit of transducin, and the coiled coil trimerization domain from cartilage matrix protein (CMPcc), respectively. Heteronuclear two-dimensional NMR spectra are used for initial peptide characterization.
000030442 536__ $$0G:(DE-Juel1)FUEK255$$2G:(DE-HGF)$$aNeurowissenschaften$$cL01$$x0
000030442 588__ $$aDataset connected to Web of Science
000030442 650_7 $$2WoSType$$aJ
000030442 65320 $$2Author$$aE. coli
000030442 65320 $$2Author$$afusion protein
000030442 65320 $$2Author$$aisotope labeling
000030442 65320 $$2Author$$aNMR
000030442 65320 $$2Author$$arecombinant peptide
000030442 7001_ $$0P:(DE-HGF)0$$aRogowski, M.$$b1
000030442 7001_ $$0P:(DE-HGF)0$$aLouis, J. M.$$b2
000030442 773__ $$0PERI:(DE-600)2006645-4$$a10.1023/A:1023887412387$$gVol. 26, p. 193 - 202$$p193 - 202$$q26<193 - 202$$tJournal of biomolecular NMR$$v26$$x0925-2738$$y2003
000030442 8567_ $$uhttp://dx.doi.org/10.1023/A:1023887412387
000030442 909CO $$ooai:juser.fz-juelich.de:30442$$pVDB
000030442 9131_ $$0G:(DE-Juel1)FUEK255$$bLeben$$kL01$$lFunktion und Dysfunktion des Nervensystems$$vNeurowissenschaften$$x0
000030442 9141_ $$y2003
000030442 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000030442 9201_ $$0I:(DE-Juel1)VDB58$$d31.12.2006$$gIBI$$kIBI-2$$lBiologische Strukturforschung$$x0
000030442 970__ $$aVDB:(DE-Juel1)28864
000030442 980__ $$aVDB
000030442 980__ $$aConvertedRecord
000030442 980__ $$ajournal
000030442 980__ $$aI:(DE-Juel1)ISB-2-20090406
000030442 980__ $$aUNRESTRICTED
000030442 980__ $$aI:(DE-Juel1)ICS-6-20110106
000030442 981__ $$aI:(DE-Juel1)IBI-7-20200312
000030442 981__ $$aI:(DE-Juel1)ISB-2-20090406
000030442 981__ $$aI:(DE-Juel1)ICS-6-20110106