TY  - JOUR
AU  - Koenig, B. W.
AU  - Rogowski, M.
AU  - Louis, J. M.
TI  - A rapid method to attain isotope labeled small soluble peptides for NMR studies
JO  - Journal of biomolecular NMR
VL  - 26
SN  - 0925-2738
CY  - Dordrecht [u.a.]
PB  - Springer Science + Business Media B.V
M1  - PreJuSER-30442
SP  - 193 - 202
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - A widely applicable strategy is presented for efficient and rapid production of small water soluble peptides expressed as fusion proteins with the immunoglobulin-binding domain of streptococcal protein G. A simple extraction and purification scheme that includes a protease cleavage step to release the target peptide is described. The yield of authentic target peptide exceeds 10 mg per liter of culture. Production of U-C-13, N-15 and highly deuterated U-C-13, N-15 isotope labeled peptide is demonstrated for the 11 residue S2 peptide, corresponding to the C-terminus of the alpha-subunit of transducin, and the coiled coil trimerization domain from cartilage matrix protein (CMPcc), respectively. Heteronuclear two-dimensional NMR spectra are used for initial peptide characterization.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000183041200001
DO  - DOI:10.1023/A:1023887412387
UR  - https://juser.fz-juelich.de/record/30442
ER  -