% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Koenig:30442,
      author       = {Koenig, B. W. and Rogowski, M. and Louis, J. M.},
      title        = {{A} rapid method to attain isotope labeled small soluble
                      peptides for {NMR} studies},
      journal      = {Journal of biomolecular NMR},
      volume       = {26},
      issn         = {0925-2738},
      address      = {Dordrecht [u.a.]},
      publisher    = {Springer Science + Business Media B.V},
      reportid     = {PreJuSER-30442},
      pages        = {193 - 202},
      year         = {2003},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {A widely applicable strategy is presented for efficient and
                      rapid production of small water soluble peptides expressed
                      as fusion proteins with the immunoglobulin-binding domain of
                      streptococcal protein G. A simple extraction and
                      purification scheme that includes a protease cleavage step
                      to release the target peptide is described. The yield of
                      authentic target peptide exceeds 10 mg per liter of culture.
                      Production of U-C-13, N-15 and highly deuterated U-C-13,
                      N-15 isotope labeled peptide is demonstrated for the 11
                      residue S2 peptide, corresponding to the C-terminus of the
                      alpha-subunit of transducin, and the coiled coil
                      trimerization domain from cartilage matrix protein (CMPcc),
                      respectively. Heteronuclear two-dimensional NMR spectra are
                      used for initial peptide characterization.},
      keywords     = {J (WoSType)},
      cin          = {IBI-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB58},
      pnm          = {Neurowissenschaften},
      pid          = {G:(DE-Juel1)FUEK255},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Spectroscopy},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000183041200001},
      doi          = {10.1023/A:1023887412387},
      url          = {https://juser.fz-juelich.de/record/30442},
}