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| Hauptseite > Publikationsdatenbank > A rapid method to attain isotope labeled small soluble peptides for NMR studies > print |
| Hauptseite > Publikationsdatenbank > A rapid method to attain isotope labeled small soluble peptides for NMR studies > print |
| 001 | 30442 | ||
| 005 | 20200402210520.0 | ||
| 024 | 7 | _ | |2 DOI |a 10.1023/A:1023887412387 |
| 024 | 7 | _ | |2 WOS |a WOS:000183041200001 |
| 037 | _ | _ | |a PreJuSER-30442 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 084 | _ | _ | |2 WoS |a Biochemistry & Molecular Biology |
| 084 | _ | _ | |2 WoS |a Spectroscopy |
| 100 | 1 | _ | |a Koenig, B. W. |b 0 |u FZJ |0 P:(DE-Juel1)132009 |
| 245 | _ | _ | |a A rapid method to attain isotope labeled small soluble peptides for NMR studies |
| 260 | _ | _ | |a Dordrecht [u.a.] |b Springer Science + Business Media B.V |c 2003 |
| 300 | _ | _ | |a 193 - 202 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of Biomolecular NMR |x 0925-2738 |0 9165 |v 26 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a A widely applicable strategy is presented for efficient and rapid production of small water soluble peptides expressed as fusion proteins with the immunoglobulin-binding domain of streptococcal protein G. A simple extraction and purification scheme that includes a protease cleavage step to release the target peptide is described. The yield of authentic target peptide exceeds 10 mg per liter of culture. Production of U-C-13, N-15 and highly deuterated U-C-13, N-15 isotope labeled peptide is demonstrated for the 11 residue S2 peptide, corresponding to the C-terminus of the alpha-subunit of transducin, and the coiled coil trimerization domain from cartilage matrix protein (CMPcc), respectively. Heteronuclear two-dimensional NMR spectra are used for initial peptide characterization. |
| 536 | _ | _ | |a Neurowissenschaften |c L01 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK255 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a E. coli |
| 653 | 2 | 0 | |2 Author |a fusion protein |
| 653 | 2 | 0 | |2 Author |a isotope labeling |
| 653 | 2 | 0 | |2 Author |a NMR |
| 653 | 2 | 0 | |2 Author |a recombinant peptide |
| 700 | 1 | _ | |a Rogowski, M. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Louis, J. M. |b 2 |0 P:(DE-HGF)0 |
| 773 | _ | _ | |a 10.1023/A:1023887412387 |g Vol. 26, p. 193 - 202 |p 193 - 202 |q 26<193 - 202 |0 PERI:(DE-600)2006645-4 |t Journal of biomolecular NMR |v 26 |y 2003 |x 0925-2738 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1023/A:1023887412387 |
| 909 | C | O | |o oai:juser.fz-juelich.de:30442 |p VDB |
| 913 | 1 | _ | |k L01 |v Neurowissenschaften |l Funktion und Dysfunktion des Nervensystems |b Leben |0 G:(DE-Juel1)FUEK255 |x 0 |
| 914 | 1 | _ | |y 2003 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k IBI-2 |l Biologische Strukturforschung |d 31.12.2006 |g IBI |0 I:(DE-Juel1)VDB58 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)28864 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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