%0 Journal Article
%A Mallick, N.
%A Mohn, F. H.
%T Use of chlorophyll fluorescence in metal-stress research: a case study with the green microalga Scenedesmus
%J Ecotoxicology and environmental safety
%V 55
%@ 0147-6513
%C Amsterdam
%I Elsevier
%M PreJuSER-31243
%P 64 - 69
%D 2003
%Z Record converted from VDB: 12.11.2012
%X Metal toxicity on the photosystem 11 (PS 11) photochemistry of the green microalga Scenedesmus obliquus was investigated in vivo using a pulse-amplitude-modulated fluorometer. The results demonstrated that the test metals (copper, chromium, nickel, cadmium, and zinc) inhibited PS II photochemistry substantially, which was clearly evident for F-0, F-v/F-m, qN, and qP following 12 h of metal exposure, whereas F-m, F-v/2, and F-0/F-v depicted significant alteration after only I h of treatment. Thus, metals are not only found to affect the initial and maximal fluorescence, maximum quantum yield, photochemical and nonphotochemical quenching, and plastoquionone pool but the water-splitting apparatus was also significantly altered under metal stress. Among all these fluorescence characteristics, the F-0/F-v registered the highest sensitivity to all the five test metals, thus indicating that the water-splitting apparatus of the oxidizing side of PS II is the primary site of action of toxic metals. This study therefore, concludes that F-0/F-v ratio can be used as a powerful tool in metal-stress research. (C) 2003 Elsevier Science (USA). All rights reserved.
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000182637200008
%R 10.1016/S0147-6513(02)00122-7
%U https://juser.fz-juelich.de/record/31243