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000031243 084__ $$2WoS$$aEnvironmental Sciences
000031243 084__ $$2WoS$$aToxicology
000031243 1001_ $$0P:(DE-HGF)0$$aMallick, N.$$b0
000031243 245__ $$aUse of chlorophyll fluorescence in metal-stress research: a case study with the green microalga Scenedesmus
000031243 260__ $$aAmsterdam$$bElsevier$$c2003
000031243 300__ $$a64 - 69
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000031243 520__ $$aMetal toxicity on the photosystem 11 (PS 11) photochemistry of the green microalga Scenedesmus obliquus was investigated in vivo using a pulse-amplitude-modulated fluorometer. The results demonstrated that the test metals (copper, chromium, nickel, cadmium, and zinc) inhibited PS II photochemistry substantially, which was clearly evident for F-0, F-v/F-m, qN, and qP following 12 h of metal exposure, whereas F-m, F-v/2, and F-0/F-v depicted significant alteration after only I h of treatment. Thus, metals are not only found to affect the initial and maximal fluorescence, maximum quantum yield, photochemical and nonphotochemical quenching, and plastoquionone pool but the water-splitting apparatus was also significantly altered under metal stress. Among all these fluorescence characteristics, the F-0/F-v registered the highest sensitivity to all the five test metals, thus indicating that the water-splitting apparatus of the oxidizing side of PS II is the primary site of action of toxic metals. This study therefore, concludes that F-0/F-v ratio can be used as a powerful tool in metal-stress research. (C) 2003 Elsevier Science (USA). All rights reserved.
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000031243 65320 $$2Author$$achlorophyll fluorescence
000031243 65320 $$2Author$$ametal stress
000031243 65320 $$2Author$$aScenedesmus
000031243 7001_ $$0P:(DE-Juel1)VDB10697$$aMohn, F. H.$$b1$$uFZJ
000031243 773__ $$0PERI:(DE-600)1466969-9$$a10.1016/S0147-6513(02)00122-7$$gVol. 55, p. 64 - 69$$p64 - 69$$q55<64 - 69$$tEcotoxicology and environmental safety$$v55$$x0147-6513$$y2003
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