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| 001 | 313 | ||
| 005 | 20200402205333.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:18585368 |
| 024 | 7 | _ | |2 DOI |a 10.1016/j.bbrc.2008.06.059 |
| 024 | 7 | _ | |2 WOS |a WOS:000258208500008 |
| 037 | _ | _ | |a PreJuSER-313 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 084 | _ | _ | |2 WoS |a Biochemistry & Molecular Biology |
| 084 | _ | _ | |2 WoS |a Biophysics |
| 100 | 1 | _ | |a Panza, G. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a Spontaneous and BSE-prion-seeded amyloid formation of full length recombinant bovine prion protein |
| 260 | _ | _ | |a Orlando, Fla. |b Academic Press |c 2008 |
| 300 | _ | _ | |a 493 - 497 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Biochemical and Biophysical Research Communications |x 0006-291X |0 787 |y 4 |v 373 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a The conversion of the cellular isoform of the prion protein into the pathogenic isoform PrP(Sc) is the key event in prion diseases. The disease can occur spontaneously genetically or by infection. In earlier studies we presented an in vitro conversion system which simulates the structural transition in recPrP by varying low concentrations of SDS at constant NaCl. In the present study we adopted the conversion system from experimental Scrapie in hamster to bovine recPrP and generated amyloid fibrils. The intermediate state which is optimal for fibril formation is a soluble, beta-rich state. The system was extended using BSE-prions as seeds and led to an acceleration of fibril formation by orders of magnitude. This seeded amyloid formation assay avoids any PK-treatment, is therefore able to detect even PK-sensitive PrP(Sc) and does not require cellular components. |
| 536 | _ | _ | |a Funktion und Dysfunktion des Nervensystems |c P33 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK409 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Amyloid: biosynthesis |
| 650 | _ | 2 | |2 MeSH |a Amyloid: chemistry |
| 650 | _ | 2 | |2 MeSH |a Animals |
| 650 | _ | 2 | |2 MeSH |a Cattle |
| 650 | _ | 2 | |2 MeSH |a Cricetinae |
| 650 | _ | 2 | |2 MeSH |a Encephalopathy, Bovine Spongiform: metabolism |
| 650 | _ | 2 | |2 MeSH |a Models, Molecular |
| 650 | _ | 2 | |2 MeSH |a PrPSc Proteins: chemistry |
| 650 | _ | 2 | |2 MeSH |a PrPSc Proteins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Protein Conformation |
| 650 | _ | 2 | |2 MeSH |a Recombinant Proteins: chemistry |
| 650 | _ | 2 | |2 MeSH |a Recombinant Proteins: metabolism |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Amyloid |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a PrPSc Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Recombinant Proteins |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a BSE-prions |
| 653 | 2 | 0 | |2 Author |a seeded vs. spontaneous conversion |
| 653 | 2 | 0 | |2 Author |a amyloid fibrils |
| 653 | 2 | 0 | |2 Author |a pre-amyloid state |
| 700 | 1 | _ | |a Stöhr, J. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Dumpitak, C. |b 2 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Papathanassiou, D. |b 3 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Weiss, J. |b 4 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Riesner, D. |b 5 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Willbold, D. |b 6 |u FZJ |0 P:(DE-Juel1)132029 |
| 700 | 1 | _ | |a Birkmann, E. |b 7 |u FZJ |0 P:(DE-Juel1)VDB65870 |
| 773 | _ | _ | |a 10.1016/j.bbrc.2008.06.059 |g Vol. 373, p. 493 - 497 |p 493 - 497 |q 373<493 - 497 |0 PERI:(DE-600)1461396-7 |t Biochemical and biophysical research communications |v 373 |y 2008 |x 0006-291X |
| 856 | 7 | _ | |u http://dx.doi.org/10.1016/j.bbrc.2008.06.059 |
| 909 | C | O | |o oai:juser.fz-juelich.de:313 |p VDB |
| 913 | 1 | _ | |k P33 |v Funktion und Dysfunktion des Nervensystems |l Funktion und Dysfunktion des Nervensystems |b Gesundheit |0 G:(DE-Juel1)FUEK409 |x 0 |
| 914 | 1 | _ | |y 2008 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k INB-2 |l Molekulare Biophysik |d 31.12.2008 |g INB |0 I:(DE-Juel1)VDB805 |x 0 |
| 920 | 1 | _ | |k JARA-SIM |l Jülich-Aachen Research Alliance - Simulation Sciences |g JARA |0 I:(DE-Juel1)VDB1045 |x 1 |
| 970 | _ | _ | |a VDB:(DE-Juel1)100579 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 980 | _ | _ | |a I:(DE-Juel1)VDB1045 |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 981 | _ | _ | |a I:(DE-Juel1)VDB1045 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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