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000031323 084__ $$2WoS$$aBiochemistry & Molecular Biology
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000031323 1001_ $$0P:(DE-HGF)0$$aJanowitz, T.$$b0
000031323 245__ $$aIdentification and characterization of plant agmatine iminohydrolase, the last missing link in polyamine biosynthesis of plants
000031323 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2003
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000031323 520__ $$aThe cloning, expression and characterization of plant agmatine iminohydrolase (AIH, also known as agmatine deiminase, EC 3.5.3.12) is described. Recombinant AIH of Arabidopsis thaliana forms dimers and catalyzes the specific conversion of agmatine to N-carbamoylputrescine and ammonia. Biochemical data suggested that cysteine side chains are involved in catalysis. However, site-directed mutagenesis of the two highly conserved cysteine residues of AIH showed that these cysteines are important but not essential for activity, arguing against a thioester substrate-enzyme intermediate during catalysis. This work represents the completion of the cloning of the arginine decarboxylase pathway genes of higher plants. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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000031323 65320 $$2Author$$aagmatine intinohydrolase (agmatine deiminase)
000031323 65320 $$2Author$$aN-carbamoylputrescine
000031323 65320 $$2Author$$apolyamine biosynthesis
000031323 65320 $$2Author$$aputrescine
000031323 65320 $$2Author$$aArabidopsis thaliana
000031323 7001_ $$0P:(DE-Juel1)VDB4417$$aKneifel, H.$$b1$$uFZJ
000031323 7001_ $$0P:(DE-HGF)0$$aPiotrowski, M.$$b2
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