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@ARTICLE{Kastenholz:31647,
      author       = {Kastenholz, B.},
      title        = {{P}reparative native continuous polyacrylamide gel
                      electrophoresis ({PNC}-page): {A}n efficient method for
                      isolating cadmium cofactors in biological systems},
      journal      = {Analytical letters},
      volume       = {37},
      issn         = {0003-2719},
      address      = {New York, NY},
      publisher    = {Taylor $\&$ Francis},
      reportid     = {PreJuSER-31647},
      pages        = {657 - 665},
      year         = {2004},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {A new electrophoretic method [preparative native continuous
                      polyacrylamide gel electrophoresis (PNC-PAGE)] was developed
                      to isolate cadmium cofactor-containing proteins in
                      biological systems.. For this purpose Arabidopsis cytosol
                      was subjected to gel permeation chromatography (GPC) and
                      high molecular mass cadmium proteins (MW approximate to 200
                      kDa) in a GPC fraction of, this Plant Were isolated by
                      PNC-PAGE. Furthermore plant cytosol was directly separated
                      by this method. The cadmium concentrations in all GPC and
                      PAGE fractions were determined by GF-AAS using matrix
                      modifier. As electrophoresis buffer of the PAGE method a
                      Tris-HCl buffer (20 mM Tris; 1 mM NaN3, pH 10.00) was used.
                      The gel (degree of polymerization of the polyacrylamide:
                      $4\%;$ gel length: 4 cm) was polymerized for 69 hr. By these
                      procedures it was revealed that the high molecular mass
                      cadmium proteins of Arabidopsis can be detected in
                      quantitative amounts by using PNC-PAGE. It could be shown
                      that the chemical structure of the cadmium proteins did not
                      change under these PAGE conditions. PNC-PAGE is supposed to
                      be an efficient method for isolating other metal cofactors
                      such as Zn, Cu, Ni, Pd, Co, Fe, Mn, Pt, Cr, and Mo and might
                      be suitable for subsequent structural determinations of
                      metalloproteins present in PAGE fractions.},
      keywords     = {J (WoSType)},
      cin          = {ICG-III},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB49},
      pnm          = {Chemie und Dynamik der Geo-Biosphäre},
      pid          = {G:(DE-Juel1)FUEK257},
      shelfmark    = {Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000220721100007},
      doi          = {10.1081/AL-120029742},
      url          = {https://juser.fz-juelich.de/record/31647},
}