Hauptseite > Publikationsdatenbank > Involvement of integrins in osmosensing and signaling towards autophagic proteolysis in rat liver > print

001     32540
005     20200423203555.0
024 7 _ |a pmid:12721289
|2 pmid
024 7 _ |a 10.1074/jbc.M210699200
|2 DOI
024 7 _ |a WOS:000184155700102
|2 WOS
024 7 _ |a 2128/2651
|2 Handle
037 _ _ |a PreJuSER-32540
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
100 1 _ |a vom Dahl, S.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Involvement of integrins in osmosensing and signaling towards autophagic proteolysis in rat liver
260 _ _ |a Bethesda, Md.
|b Soc.
|c 2003
300 _ _ |a 27088 - 27095
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Journal of Biological Chemistry
|x 0021-9258
|0 3091
|v 278
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Inhibition of autophagic proteolysis by hypoosmotic or amino acid-induced hepatocyte swelling requires osmosignaling toward p38MAPK; however, the upstream osmosensing and signaling events are unknown. These were studied in the intact perfused rat liver with a preserved in situ environment of hepatocytes. It was found that hypoosmotic hepatocyte swelling led to an activation of Src (but not FAK), Erks, and p38MAPK, which was prevented by the integrin inhibitory hexapeptide GRGDSP, but not its inactive analogue GRGESP. Src inhibition by PP-2 prevented hypoosmotic MAP kinase activation, indicating that the integrin/Src system is located upstream in the osmosignaling toward p38MAPK and Erks. Inhibition of the integrin/Src system by the RGD motif-containing peptide or PP-2 also prevented the inhibition of proteolysis and the decrease in autophagic vacuole volume, which is otherwise observed in response to hypoosmotic or glutamine/glycine-induced hepatocyte swelling. These inhibitors, however, did not affect swelling-independent proteolysis inhibition by phenylalanine. In line with a role of p38MAPK in triggering the volume regulatory decrease (RVD), PP-2 and the RGD peptide blunted RVD in response to hypoosmotic cell swelling. The data identify integrins and Src as upstream events in the osmosignaling toward MAP kinases, proteolysis, and RVD. They further point to a role of integrins as osmo- and mechanosensors in the intact liver, which may provide a link between cell volume and cell function.
536 _ _ |a Neurowissenschaften
|c L01
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK255
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Autophagy
650 _ 2 |2 MeSH
|a Cell Size: drug effects
650 _ 2 |2 MeSH
|a Cell Size: physiology
650 _ 2 |2 MeSH
|a Cells, Cultured
650 _ 2 |2 MeSH
|a Hepatocytes: cytology
650 _ 2 |2 MeSH
|a Hepatocytes: drug effects
650 _ 2 |2 MeSH
|a Hepatocytes: physiology
650 _ 2 |2 MeSH
|a Integrins: antagonists & inhibitors
650 _ 2 |2 MeSH
|a Integrins: physiology
650 _ 2 |2 MeSH
|a Liver: drug effects
650 _ 2 |2 MeSH
|a Liver: physiology
650 _ 2 |2 MeSH
|a Liver: ultrastructure
650 _ 2 |2 MeSH
|a MAP Kinase Signaling System
650 _ 2 |2 MeSH
|a Male
650 _ 2 |2 MeSH
|a Mitogen-Activated Protein Kinases: metabolism
650 _ 2 |2 MeSH
|a Models, Biological
650 _ 2 |2 MeSH
|a Oligopeptides: pharmacology
650 _ 2 |2 MeSH
|a Perfusion
650 _ 2 |2 MeSH
|a Rats
650 _ 2 |2 MeSH
|a Rats, Wistar
650 _ 2 |2 MeSH
|a Signal Transduction
650 _ 2 |2 MeSH
|a Water-Electrolyte Balance: physiology
650 _ 2 |2 MeSH
|a p38 Mitogen-Activated Protein Kinases
650 _ 2 |2 MeSH
|a src-Family Kinases: antagonists & inhibitors
650 _ 2 |2 MeSH
|a src-Family Kinases: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Integrins
650 _ 7 |0 0
|2 NLM Chemicals
|a Oligopeptides
650 _ 7 |0 91037-75-1
|2 NLM Chemicals
|a glycyl-arginyl-glycyl-aspartyl-seryl-proline
650 _ 7 |0 99896-85-2
|2 NLM Chemicals
|a arginyl-glycyl-aspartic acid
650 _ 7 |0 99896-88-5
|2 NLM Chemicals
|a glycyl-arginyl-glycyl-glutamyl-seryl-proline
650 _ 7 |0 EC 2.7.10.2
|2 NLM Chemicals
|a src-Family Kinases
650 _ 7 |0 EC 2.7.11.24
|2 NLM Chemicals
|a Mitogen-Activated Protein Kinases
650 _ 7 |0 EC 2.7.11.24
|2 NLM Chemicals
|a p38 Mitogen-Activated Protein Kinases
650 _ 7 |a J
|2 WoSType
700 1 _ |a Schliess, F.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Reissmann, R.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Görg, B.
|b 3
|0 P:(DE-HGF)0
700 1 _ |a Weiergräber, O.
|b 4
|u FZJ
|0 P:(DE-Juel1)131988
700 1 _ |a Kocalkova, M.
|b 5
|0 P:(DE-HGF)0
700 1 _ |a Dombrowski, F.
|b 6
|0 P:(DE-HGF)0
700 1 _ |a Häussinger, D.
|b 7
|0 P:(DE-HGF)0
773 _ _ |a 10.1074/jbc.M210699200
|g Vol. 278, p. 27088 - 27095
|p 27088 - 27095
|q 278<27088 - 27095
|0 PERI:(DE-600)1474604-9
|t The @journal of biological chemistry
|v 278
|y 2003
|x 0021-9258
856 7 _ |u http://dx.doi.org/10.1074/jbc.M210699200
|u http://hdl.handle.net/2128/2651
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