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000032554 0247_ $$2pmid$$apmid:14745783
000032554 0247_ $$2DOI$$a10.1002/yea.1058
000032554 0247_ $$2WOS$$aWOS:000188635600006
000032554 037__ $$aPreJuSER-32554
000032554 041__ $$aeng
000032554 082__ $$a570
000032554 084__ $$2WoS$$aBiochemistry & Molecular Biology
000032554 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000032554 084__ $$2WoS$$aMicrobiology
000032554 084__ $$2WoS$$aMycology
000032554 1001_ $$0P:(DE-Juel1)VDB2840$$aSchlösser, T.$$b0$$uFZJ
000032554 245__ $$aAlanine: glyoxylate aminotransferase of Saccharomyces cerevisiae - encoding gene AGX1 and metabolic significance
000032554 260__ $$aNew York, NY [u.a.]$$bWiley$$c2004
000032554 300__ $$a63 - 73
000032554 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000032554 3367_ $$00$$2EndNote$$aJournal Article
000032554 3367_ $$2BibTeX$$aARTICLE
000032554 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000032554 3367_ $$2DRIVER$$aarticle
000032554 440_0 $$09023$$aYeast$$v21$$x0749-503X$$y1
000032554 500__ $$aRecord converted from VDB: 12.11.2012
000032554 520__ $$aAlanine : glyoxylate aminotransferase is one of three different enzymes used for glycine synthesis in Saccharomyces cerevisiae. The open reading frame YFL030w (named AGX1 in the following), encoding this enzyme, was identified by comparing enzyme specific activities in knockout strains. While 100% activity was detectable in the parental strain, 2% was found in a YFL030w::kanMX4 strain. The ORF found at that locus was suspected to encode alanine : glyoxylate aminotransferase because its predicted amino acid sequence showed 23% identity to the human homologue. Since the YFL030w::kanMX4 strain showed no glycine auxtrophic phenotype, AGX1 was replaced by KanMX4 in a Delta GLY1 Delta SHM1 Delta SHM2 background. These background mutations, which cause inactivation of threonine aldolase, mitochondrial and cytosolic serine hydroxymethyltransferase, respectively, lead to a conditional glycine auxotrophy. This means that growth is not possible on glucose but on ethanol as the sole carbon source. Additional disruption of AGX1 revealed a complete glycine auxotrophy. Complementation was observed by transformation with a plasmid-encoded AGX1.
000032554 536__ $$0G:(DE-Juel1)FUEK256$$2G:(DE-HGF)$$aBiotechnologie$$cL02$$x0
000032554 588__ $$aDataset connected to Web of Science, Pubmed
000032554 650_2 $$2MeSH$$aAlanine: metabolism
000032554 650_2 $$2MeSH$$aAmino Acid Sequence
000032554 650_2 $$2MeSH$$aAnimals
000032554 650_2 $$2MeSH$$aBase Sequence
000032554 650_2 $$2MeSH$$aDNA, Fungal: chemistry
000032554 650_2 $$2MeSH$$aDNA, Fungal: genetics
000032554 650_2 $$2MeSH$$aGlycine: biosynthesis
000032554 650_2 $$2MeSH$$aGlyoxylates: metabolism
000032554 650_2 $$2MeSH$$aHumans
000032554 650_2 $$2MeSH$$aModels, Chemical
000032554 650_2 $$2MeSH$$aMolecular Sequence Data
000032554 650_2 $$2MeSH$$aMutagenesis, Insertional
000032554 650_2 $$2MeSH$$aOrotic Acid: analogs & derivatives
000032554 650_2 $$2MeSH$$aOrotic Acid: metabolism
000032554 650_2 $$2MeSH$$aSaccharomyces cerevisiae: enzymology
000032554 650_2 $$2MeSH$$aSaccharomyces cerevisiae: genetics
000032554 650_2 $$2MeSH$$aSelection, Genetic
000032554 650_2 $$2MeSH$$aSequence Alignment
000032554 650_2 $$2MeSH$$aTransaminases: genetics
000032554 650_2 $$2MeSH$$aTransaminases: metabolism
000032554 650_7 $$00$$2NLM Chemicals$$aDNA, Fungal
000032554 650_7 $$00$$2NLM Chemicals$$aGlyoxylates
000032554 650_7 $$0298-12-4$$2NLM Chemicals$$aglyoxylic acid
000032554 650_7 $$056-40-6$$2NLM Chemicals$$aGlycine
000032554 650_7 $$056-41-7$$2NLM Chemicals$$aAlanine
000032554 650_7 $$065-86-1$$2NLM Chemicals$$aOrotic Acid
000032554 650_7 $$0703-95-7$$2NLM Chemicals$$a5-fluoroorotic acid
000032554 650_7 $$0EC 2.6.1.-$$2NLM Chemicals$$aTransaminases
000032554 650_7 $$0EC 2.6.1.44$$2NLM Chemicals$$aAlanine-glyoxylate transaminase
000032554 650_7 $$2WoSType$$aJ
000032554 65320 $$2Author$$aalanine : glyoxylate aminotransferase
000032554 65320 $$2Author$$aglycine biosynthesis
000032554 65320 $$2Author$$aamino acid metabolism
000032554 65320 $$2Author$$aSaccharomyces cerevisiae
000032554 65320 $$2Author$$afungi
000032554 7001_ $$0P:(DE-Juel1)VDB2847$$aGätgens, C.$$b1$$uFZJ
000032554 7001_ $$0P:(DE-Juel1)VDB2942$$aWeber, U.$$b2$$uFZJ
000032554 7001_ $$0P:(DE-Juel1)VDB12831$$aStahmann, K. P.$$b3$$uFZJ
000032554 773__ $$0PERI:(DE-600)1479172-9$$a10.1002/yea.1058$$gVol. 21, p. 63 - 73$$p63 - 73$$q21<63 - 73$$tYeast$$v21$$x0749-503X$$y2004
000032554 8567_ $$uhttp://dx.doi.org/10.1002/yea.1058
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000032554 9131_ $$0G:(DE-Juel1)FUEK256$$bLeben$$kL02$$lBiotechnologie$$vBiotechnologie$$x0
000032554 9141_ $$y2004
000032554 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000032554 9201_ $$0I:(DE-Juel1)VDB55$$gIBT$$kIBT-1$$lBiotechnologie 1$$x0$$zab 31.10.10 weitergeführt als IBG-1
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