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@ARTICLE{Yu:33246,
      author       = {Yu, T.-W. and Müller, R. and Müller, M. and Floss, H. G.},
      title        = {{M}utational analysis and reconstituted expression of the
                      biosynthetic genes involved in the formation of
                      3-amino-5-hydroxybenzoic acid : the starter unit of
                      rifamycin biosynthesis in {A}mycolatopsis mediterranei
                      {S}699},
      journal      = {The journal of biological chemistry},
      volume       = {276},
      issn         = {0021-9258},
      address      = {Bethesda, Md.},
      publisher    = {Soc.},
      reportid     = {PreJuSER-33246},
      pages        = {12546 - 12555},
      year         = {2001},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {To investigate a novel branch of the shikimate biosynthesis
                      pathway operating in the formation of
                      3-amino-5-hydroxybenzoic acid (AHBA), the unique
                      biosynthetic precursor of rifamycin and related ansamycins,
                      a series of target-directed mutations and heterologous gene
                      expressions were investigated in Amycolatopsis mediterranei
                      and Streptomyces coelicolor. The genes involved in AHBA
                      formation were inactivated individually, and the resulting
                      mutants were further examined by incubating the cell-free
                      extracts with known intermediates of the pathway and
                      analyzing for AHBA formation. The rifL, -M, and -N genes
                      were shown to be involved in the step(s) from either
                      phosphoenolpyruvate/d-erythrose 4-phosphate or other
                      precursors to 3,4-dideoxy-4-amino-d-arabino-heptulosonate
                      7-phosphate. The gene products of the rifH, -G, and -J genes
                      resemble enzymes involved in the shikimate biosynthesis
                      pathway (August, P. R., Tang, L., Yoon, Y. J., Ning, S.,
                      Müller, R., Yu, T.-W., Taylor, M., Hoffmann, D., Kim,
                      C.-G., Zhang, X., Hutchinson, C. R., and Floss, H. G. (1998)
                      Chem. Biol. 5, 69-79). Mutants of the rifH and -J genes
                      produced rifamycin B at $1\%$ and $10\%,$ respectively, of
                      the yields of the wild type; inactivation of the rifG gene
                      did not affect rifamycin production significantly. Finally,
                      coexpressing the rifG-N and -J genes in S. coelicolor YU105
                      under the control of the act promoter led to significant
                      production of AHBA in the fermented cultures, confirming
                      that seven of these genes are indeed necessary and
                      sufficient for AHBA formation. The effects of deletion of
                      individual genes from the heterologous expression cassette
                      on AHBA formation duplicated the effects of the genomic
                      rifG-N and -J mutations on rifamycin production, indicating
                      that all these genes encode proteins with catalytic rather
                      than regulatory functions in AHBA formation for rifamycin
                      biosynthesis by A. mediterranei.},
      keywords     = {Actinomycetales: enzymology / Actinomycetales: genetics /
                      Amino Acid Sequence / Aminobenzoic Acids: metabolism / Base
                      Sequence / DNA Mutational Analysis: methods / DNA Primers /
                      Genes, Bacterial / Genes, Regulator / Molecular Sequence
                      Data / Multigene Family / Open Reading Frames / Plasmids /
                      Promoter Regions, Genetic / Restriction Mapping / Rifabutin:
                      metabolism / Rifamycins: biosynthesis / Aminobenzoic Acids
                      (NLM Chemicals) / DNA Primers (NLM Chemicals) / Rifamycins
                      (NLM Chemicals) / Rifabutin (NLM Chemicals) /
                      3-amino-5-hydroxybenzoic acid (NLM Chemicals) / J (WoSType)},
      cin          = {IBT-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Verfahrenstechnik zur mikrobiellen Gewinnung von
                      Primärmetaboliten},
      pid          = {G:(DE-Juel1)FUEK93},
      shelfmark    = {Biochemistry $\&$ Molecular Biology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:11278540},
      UT           = {WOS:000168198600013},
      doi          = {10.1074/jbc.M009667200},
      url          = {https://juser.fz-juelich.de/record/33246},
}