Home > Publications database > Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid : the starter unit of rifamycin biosynthesis in Amycolatopsis mediterranei S699 > print

001     33246
005     20200423203609.0
024 7 _ |a pmid:11278540
|2 pmid
024 7 _ |a 10.1074/jbc.M009667200
|2 DOI
024 7 _ |a WOS:000168198600013
|2 WOS
024 7 _ |a 2128/2835
|2 Handle
024 7 _ |a altmetric:21810741
|2 altmetric
037 _ _ |a PreJuSER-33246
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
100 1 _ |a Yu, T.-W.
|0 P:(DE-HGF)0
|b 0
245 _ _ |a Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid : the starter unit of rifamycin biosynthesis in Amycolatopsis mediterranei S699
260 _ _ |a Bethesda, Md.
|b Soc.
|c 2001
300 _ _ |a 12546 - 12555
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Journal of Biological Chemistry
|x 0021-9258
|0 3091
|y 16
|v 276
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a To investigate a novel branch of the shikimate biosynthesis pathway operating in the formation of 3-amino-5-hydroxybenzoic acid (AHBA), the unique biosynthetic precursor of rifamycin and related ansamycins, a series of target-directed mutations and heterologous gene expressions were investigated in Amycolatopsis mediterranei and Streptomyces coelicolor. The genes involved in AHBA formation were inactivated individually, and the resulting mutants were further examined by incubating the cell-free extracts with known intermediates of the pathway and analyzing for AHBA formation. The rifL, -M, and -N genes were shown to be involved in the step(s) from either phosphoenolpyruvate/d-erythrose 4-phosphate or other precursors to 3,4-dideoxy-4-amino-d-arabino-heptulosonate 7-phosphate. The gene products of the rifH, -G, and -J genes resemble enzymes involved in the shikimate biosynthesis pathway (August, P. R., Tang, L., Yoon, Y. J., Ning, S., Müller, R., Yu, T.-W., Taylor, M., Hoffmann, D., Kim, C.-G., Zhang, X., Hutchinson, C. R., and Floss, H. G. (1998) Chem. Biol. 5, 69-79). Mutants of the rifH and -J genes produced rifamycin B at 1% and 10%, respectively, of the yields of the wild type; inactivation of the rifG gene did not affect rifamycin production significantly. Finally, coexpressing the rifG-N and -J genes in S. coelicolor YU105 under the control of the act promoter led to significant production of AHBA in the fermented cultures, confirming that seven of these genes are indeed necessary and sufficient for AHBA formation. The effects of deletion of individual genes from the heterologous expression cassette on AHBA formation duplicated the effects of the genomic rifG-N and -J mutations on rifamycin production, indicating that all these genes encode proteins with catalytic rather than regulatory functions in AHBA formation for rifamycin biosynthesis by A. mediterranei.
536 _ _ |a Verfahrenstechnik zur mikrobiellen Gewinnung von Primärmetaboliten
|c 41.40.0
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK93
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Actinomycetales: enzymology
650 _ 2 |2 MeSH
|a Actinomycetales: genetics
650 _ 2 |2 MeSH
|a Amino Acid Sequence
650 _ 2 |2 MeSH
|a Aminobenzoic Acids: metabolism
650 _ 2 |2 MeSH
|a Base Sequence
650 _ 2 |2 MeSH
|a DNA Mutational Analysis: methods
650 _ 2 |2 MeSH
|a DNA Primers
650 _ 2 |2 MeSH
|a Genes, Bacterial
650 _ 2 |2 MeSH
|a Genes, Regulator
650 _ 2 |2 MeSH
|a Molecular Sequence Data
650 _ 2 |2 MeSH
|a Multigene Family
650 _ 2 |2 MeSH
|a Open Reading Frames
650 _ 2 |2 MeSH
|a Plasmids
650 _ 2 |2 MeSH
|a Promoter Regions, Genetic
650 _ 2 |2 MeSH
|a Restriction Mapping
650 _ 2 |2 MeSH
|a Rifabutin: metabolism
650 _ 2 |2 MeSH
|a Rifamycins: biosynthesis
650 _ 7 |0 0
|2 NLM Chemicals
|a Aminobenzoic Acids
650 _ 7 |0 0
|2 NLM Chemicals
|a DNA Primers
650 _ 7 |0 0
|2 NLM Chemicals
|a Rifamycins
650 _ 7 |0 72559-06-9
|2 NLM Chemicals
|a Rifabutin
650 _ 7 |0 76045-71-1
|2 NLM Chemicals
|a 3-amino-5-hydroxybenzoic acid
650 _ 7 |a J
|2 WoSType
700 1 _ |a Müller, R.
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Müller, M.
|0 P:(DE-Juel1)VDB1106
|b 2
|u FZJ
700 1 _ |a Floss, H. G.
|0 P:(DE-HGF)0
|b 3
773 _ _ |a 10.1074/jbc.M009667200
|g Vol. 276, p. 12546 - 12555
|p 12546 - 12555
|q 276<12546 - 12555
|0 PERI:(DE-600)1474604-9
|t The @journal of biological chemistry
|v 276
|y 2001
|x 0021-9258
856 4 _ |u https://juser.fz-juelich.de/record/33246/files/3728.pdf
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/33246/files/3728.jpg?subformat=icon-1440
|x icon-1440
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/33246/files/3728.jpg?subformat=icon-180
|x icon-180
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/33246/files/3728.jpg?subformat=icon-640
|x icon-640
|y OpenAccess
909 C O |o oai:juser.fz-juelich.de:33246
|p openaire
|p open_access
|p driver
|p VDB
|p dnbdelivery
913 1 _ |k 41.40.0
|v Verfahrenstechnik zur mikrobiellen Gewinnung von Primärmetaboliten
|l Biotechnologie
|b Lebenswissenschaften
|0 G:(DE-Juel1)FUEK93
|x 0
914 1 _ |y 2001
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
915 _ _ |2 StatID
|0 StatID:(DE-HGF)0510
|a OpenAccess
920 1 _ |k IBT-2
|l Biotechnologie 2
|g IBT
|z ab 31.10.10 weitergeführt IBG-1
|0 I:(DE-Juel1)VDB56
|x 0
970 _ _ |a VDB:(DE-Juel1)3728
980 _ _ |a VDB
980 _ _ |a JUWEL
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)IBG-1-20101118
980 _ _ |a UNRESTRICTED
980 _ _ |a FullTexts
980 1 _ |a FullTexts
981 _ _ |a I:(DE-Juel1)IBG-1-20101118

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21