TY - THES
AU - Wolfram, Evelyn P.
TI - Parallele Substratkontrolle in Bioreaktoren mit geringem Volumen
VL - 3982
IS - Juel-3982
SN - 0944-2952
PB - Techn. Univ. München
VL - Dr. (Univ.)
CY - Jülich
M1 - PreJuSER-33332
M1 - Juel-3982
T2 - Berichte des Forschungszentrums Jülich
SP - VII, 194 p.
PY - 2002
N1 - Record converted from VDB: 12.11.2012
N1 - München, Techn. Univ., Diss., 2001
AB - In the first developmental phase of microbial processes, relevant parameters for reaction engineering are investigated and optimized with parallel experiments in small reactors (shake flask scale) . Substrate concentrations are central process parameters . Their control might help to increase efficiency and quality of this developmental phase . Thus, a parallel, automatic and sterile sampling technique wich hollow fiber membranes was developed that suits the Small reaction volume. The parallel sampling modules were connected to a Substrate analysis system. The combination of the analytical technique with a controller and a precise microdosing System for Substrate closed the control loop. The prototype device was characterized and its suitability for online monitoring and control of bioprocesses confirmed . This technique allows for the firnt time online measurement and control of Substrate concentrations in parallel bioreactions (i.e . with Escherichia coli) wich measuring raten of the order of minutes
LB - PUB:(DE-HGF)11 ; PUB:(DE-HGF)3
UR - https://juser.fz-juelich.de/record/33332
ER -