TY  - JOUR
AU  - Rahmani, F.
AU  - Hummel, M.
AU  - Schuurmans, J.
AU  - Wiese-Klinkenberg, A.
AU  - Smeekens, S.
AU  - Hanson, J.
TI  - Sucrose control of translation mediated by an upstream open reading frame-encoded peptide 1[W][OA]
JO  - Plant physiology
VL  - 150
SN  - 0032-0889
CY  - Rockville, Md.: Soc.
PB  - JSTOR
M1  - PreJuSER-3375
SP  - 1356 - 1367
PY  - 2009
N1  - This work was supported by the Centre for BioSystems Genomics and by the Dutch Research Council.
AB  - Regulation of gene expression through translational control is common in many organisms. The Arabidopsis (Arabidopsis thaliana) transcription factor bZIP11 is translational repressed in response to sucrose (Suc), resulting in Suc-regulated changes in amino acid metabolism. The 5' leader of the bZIP11 mRNA harbors several upstream open reading frames (uORFs), of which the second uORF is well conserved among bZIP11 homologous genes. The uORF2 element encodes a Suc control peptide (SC-peptide) of 28 residues that is sufficient for imposing Suc-induced repression of translation (SIRT) on a heterologous mRNA. Detailed analysis of the SC-peptide suggests that it functions as an attenuator peptide. Results suggest that the SC-peptide inhibits bZIP11 translation in response to high Suc levels by stalling the ribosome on the mRNA. The conserved noncanonical AUG contexts of bZIP11 uORFs allow inefficient translational initiation of the uORF, resulting in translation initiation of the scanning ribosome at the AUG codon of the bZIP11 main ORF. The results presented show that Suc-dependent signaling mediates differential translation of mRNAs containing SC-peptides encoding uORFs.
KW  - Amino Acid Sequence
KW  - Arabidopsis: drug effects
KW  - Arabidopsis: genetics
KW  - Arabidopsis Proteins: chemistry
KW  - Arabidopsis Proteins: genetics
KW  - Arabidopsis Proteins: metabolism
KW  - Base Sequence
KW  - Basic-Leucine Zipper Transcription Factors: chemistry
KW  - Basic-Leucine Zipper Transcription Factors: genetics
KW  - Basic-Leucine Zipper Transcription Factors: metabolism
KW  - Conserved Sequence
KW  - Gene Expression Regulation, Plant: drug effects
KW  - Molecular Sequence Data
KW  - Open Reading Frames
KW  - Protein Biosynthesis: drug effects
KW  - RNA, Messenger: chemistry
KW  - Sequence Analysis, RNA
KW  - Sucrose: pharmacology
KW  - ATB2 protein, Arabidopsis (NLM Chemicals)
KW  - Arabidopsis Proteins (NLM Chemicals)
KW  - Basic-Leucine Zipper Transcription Factors (NLM Chemicals)
KW  - RNA, Messenger (NLM Chemicals)
KW  - Sucrose (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19403731
C2  - pmc:PMC2705056
UR  - <Go to ISI:>//WOS:000268696800021
DO  - DOI:10.1104/pp.109.136036
UR  - https://juser.fz-juelich.de/record/3375
ER  -