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@ARTICLE{Rahmani:3375,
author = {Rahmani, F. and Hummel, M. and Schuurmans, J. and
Wiese-Klinkenberg, A. and Smeekens, S. and Hanson, J.},
title = {{S}ucrose control of translation mediated by an upstream
open reading frame-encoded peptide 1[{W}][{OA}]},
journal = {Plant physiology},
volume = {150},
issn = {0032-0889},
address = {Rockville, Md.: Soc.},
publisher = {JSTOR},
reportid = {PreJuSER-3375},
pages = {1356 - 1367},
year = {2009},
note = {This work was supported by the Centre for BioSystems
Genomics and by the Dutch Research Council.},
abstract = {Regulation of gene expression through translational control
is common in many organisms. The Arabidopsis (Arabidopsis
thaliana) transcription factor bZIP11 is translational
repressed in response to sucrose (Suc), resulting in
Suc-regulated changes in amino acid metabolism. The 5'
leader of the bZIP11 mRNA harbors several upstream open
reading frames (uORFs), of which the second uORF is well
conserved among bZIP11 homologous genes. The uORF2 element
encodes a Suc control peptide (SC-peptide) of 28 residues
that is sufficient for imposing Suc-induced repression of
translation (SIRT) on a heterologous mRNA. Detailed analysis
of the SC-peptide suggests that it functions as an
attenuator peptide. Results suggest that the SC-peptide
inhibits bZIP11 translation in response to high Suc levels
by stalling the ribosome on the mRNA. The conserved
noncanonical AUG contexts of bZIP11 uORFs allow inefficient
translational initiation of the uORF, resulting in
translation initiation of the scanning ribosome at the AUG
codon of the bZIP11 main ORF. The results presented show
that Suc-dependent signaling mediates differential
translation of mRNAs containing SC-peptides encoding uORFs.},
keywords = {Amino Acid Sequence / Arabidopsis: drug effects /
Arabidopsis: genetics / Arabidopsis Proteins: chemistry /
Arabidopsis Proteins: genetics / Arabidopsis Proteins:
metabolism / Base Sequence / Basic-Leucine Zipper
Transcription Factors: chemistry / Basic-Leucine Zipper
Transcription Factors: genetics / Basic-Leucine Zipper
Transcription Factors: metabolism / Conserved Sequence /
Gene Expression Regulation, Plant: drug effects / Molecular
Sequence Data / Open Reading Frames / Protein Biosynthesis:
drug effects / RNA, Messenger: chemistry / Sequence
Analysis, RNA / Sucrose: pharmacology / ATB2 protein,
Arabidopsis (NLM Chemicals) / Arabidopsis Proteins (NLM
Chemicals) / Basic-Leucine Zipper Transcription Factors (NLM
Chemicals) / RNA, Messenger (NLM Chemicals) / Sucrose (NLM
Chemicals) / J (WoSType)},
cin = {ICG-3},
ddc = {580},
cid = {I:(DE-Juel1)ICG-3-20090406},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Plant Sciences},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19403731},
pmc = {pmc:PMC2705056},
UT = {WOS:000268696800021},
doi = {10.1104/pp.109.136036},
url = {https://juser.fz-juelich.de/record/3375},
}