000000340 001__ 340
000000340 005__ 20200402205336.0
000000340 0247_ $$2pmid$$apmid:18669653
000000340 0247_ $$2pmc$$apmc:PMC2504809
000000340 0247_ $$2DOI$$a10.1073/pnas.0804721105
000000340 0247_ $$2WOS$$aWOS:000258308500036
000000340 037__ $$aPreJuSER-340
000000340 041__ $$aeng
000000340 082__ $$a000
000000340 084__ $$2WoS$$aMultidisciplinary Sciences
000000340 1001_ $$0P:(DE-HGF)0$$aElfrink, K.$$b0
000000340 245__ $$aStructural changes of membrane-anchored native PrPc
000000340 260__ $$aWashington, DC$$bAcademy$$c2008
000000340 300__ $$a10815 - 10819
000000340 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000000340 3367_ $$2DataCite$$aOutput Types/Journal article
000000340 3367_ $$00$$2EndNote$$aJournal Article
000000340 3367_ $$2BibTeX$$aARTICLE
000000340 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000000340 3367_ $$2DRIVER$$aarticle
000000340 440_0 $$05100$$aProceedings of the National Academy of Sciences of the United States of America$$v105$$x0027-8424
000000340 500__ $$aRecord converted from VDB: 12.11.2012
000000340 520__ $$aMisfolding and subsequent aggregation of endogenous proteins constitute essential steps in many human disorders, including Alzheimer and prion diseases. In most prion protein-folding studies, the posttranslational modifications, the lipid anchor in particular, were lacking. Here, we studied a fully posttranslationally modified cellular prion protein, carrying two N-glycosylations and the natural GPI anchor. We used time-resolved FTIR to study the prion protein secondary structure changes when binding to a raft-like lipid membrane via its GPI anchor. We observed that membrane anchoring above a threshold concentration induced refolding of the prion protein to intermolecular beta-sheets. Such transition is not observed in solution and is membrane specific. Excessive membrane anchoring, analyzed with molecular sensitivity, is thought to be a crucial event in the development of prion diseases.
000000340 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000000340 588__ $$aDataset connected to Web of Science, Pubmed
000000340 650_2 $$2MeSH$$aAnimals
000000340 650_2 $$2MeSH$$aCricetinae
000000340 650_2 $$2MeSH$$aMembrane Proteins: genetics
000000340 650_2 $$2MeSH$$aMesocricetus
000000340 650_2 $$2MeSH$$aModels, Molecular
000000340 650_2 $$2MeSH$$aPrPC Proteins: genetics
000000340 650_2 $$2MeSH$$aProtein Conformation
000000340 650_2 $$2MeSH$$aProtein Folding
000000340 650_2 $$2MeSH$$aSpectroscopy, Fourier Transform Infrared
000000340 650_7 $$00$$2NLM Chemicals$$aMembrane Proteins
000000340 650_7 $$00$$2NLM Chemicals$$aPrPC Proteins
000000340 650_7 $$2WoSType$$aJ
000000340 65320 $$2Author$$aFTIR
000000340 65320 $$2Author$$amembrane anchoring
000000340 65320 $$2Author$$aprion protein
000000340 65320 $$2Author$$aprotein aggregation
000000340 65320 $$2Author$$asecondary structure
000000340 7001_ $$0P:(DE-HGF)0$$aOllesch, J.$$b1
000000340 7001_ $$0P:(DE-HGF)0$$aStoehr, J.$$b2
000000340 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b3$$uFZJ
000000340 7001_ $$0P:(DE-HGF)0$$aRiesner, D.$$b4
000000340 7001_ $$0P:(DE-HGF)0$$aGerwert, K.$$b5
000000340 773__ $$0PERI:(DE-600)1461794-8$$a10.1073/pnas.0804721105$$gVol. 105, p. 10815 - 10819$$p10815 - 10819$$q105<10815 - 10819$$tProceedings of the National Academy of Sciences of the United States of America$$v105$$x0027-8424$$y2008
000000340 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504809
000000340 909CO $$ooai:juser.fz-juelich.de:340$$pVDB
000000340 9131_ $$0G:(DE-Juel1)FUEK409$$bGesundheit$$kP33$$lFunktion und Dysfunktion des Nervensystems$$vFunktion und Dysfunktion des Nervensystems$$x0
000000340 9141_ $$y2008
000000340 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000000340 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x0
000000340 970__ $$aVDB:(DE-Juel1)100614
000000340 980__ $$aVDB
000000340 980__ $$aConvertedRecord
000000340 980__ $$ajournal
000000340 980__ $$aI:(DE-Juel1)ISB-2-20090406
000000340 980__ $$aUNRESTRICTED
000000340 980__ $$aI:(DE-Juel1)ICS-6-20110106
000000340 981__ $$aI:(DE-Juel1)IBI-7-20200312
000000340 981__ $$aI:(DE-Juel1)ISB-2-20090406
000000340 981__ $$aI:(DE-Juel1)ICS-6-20110106