%0 Journal Article
%A Curves, S.
%A Linnemann, J.
%A Klauser, T.
%A Wandrey, C.
%A Takors, R.
%T Recombinant protein production with pichia pastoris in continuous fermentation - kinetic analysis of growth and production formation
%J Chemie - Ingenieur - Technik
%V 73
%@ 0009-286X
%C Weinheim
%I Wiley-VCH Verl.
%M PreJuSER-34117
%P 1615 - 1621
%D 2001
%Z Record converted from VDB: 12.11.2012
%X Continuous fermentation was applied to the production of recombinant human chymotrypsinogen B (hCTRB) by the methylotrophic yeast Pichia pastoris as a tool for the kinetic analysis of growth and product formation. Using methanol as the sole source of carbon, energy, and induction, cell growth could be described by a non-competitive M ON approach. The maximum growth rate p a,, was determined to be 0.084 h(-1) and the K-M-value for methanol to 0.22 g L-1, respectively. With respect to product formation a similar model was established exhibiting a methanol concentration of 0.13 g L-1 as the K-M-value and a maximum biomass-specific product-formation rate Of pi(max) = 0.23 mg g(-1) h(-1). The production of hCTRB was strictly growth-coupled.The data provided covers the range of methanol concentrations between 0 and 4 g L-1. Substrate concentrations exceeding this upper value led to a complete collapse of product formation. This change in phenotype turned out to be irreversible indicating a genetic instability of transformed Pichia pastoris caused by excess methanol.
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000173288200018
%R 10.1002/1522-2640(200112)73:12<1615::AID-CITE1615>3.0.CO;2-6
%U https://juser.fz-juelich.de/record/34117