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000034117 084__ $$2WoS$$aEngineering, Chemical
000034117 1001_ $$0P:(DE-Juel1)VDB4399$$aCurves, S.$$b0$$uFZJ
000034117 245__ $$aRecombinant protein production with pichia pastoris in continuous fermentation - kinetic analysis of growth and production formation
000034117 260__ $$aWeinheim$$bWiley-VCH Verl.$$c2001
000034117 300__ $$a1615 - 1621
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000034117 440_0 $$01215$$aChemie Ingenieur Technik$$v73$$x0009-286X$$y12
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000034117 520__ $$aContinuous fermentation was applied to the production of recombinant human chymotrypsinogen B (hCTRB) by the methylotrophic yeast Pichia pastoris as a tool for the kinetic analysis of growth and product formation. Using methanol as the sole source of carbon, energy, and induction, cell growth could be described by a non-competitive M ON approach. The maximum growth rate p a,, was determined to be 0.084 h(-1) and the K-M-value for methanol to 0.22 g L-1, respectively. With respect to product formation a similar model was established exhibiting a methanol concentration of 0.13 g L-1 as the K-M-value and a maximum biomass-specific product-formation rate Of pi(max) = 0.23 mg g(-1) h(-1). The production of hCTRB was strictly growth-coupled.The data provided covers the range of methanol concentrations between 0 and 4 g L-1. Substrate concentrations exceeding this upper value led to a complete collapse of product formation. This change in phenotype turned out to be irreversible indicating a genetic instability of transformed Pichia pastoris caused by excess methanol.
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000034117 7001_ $$0P:(DE-HGF)0$$aLinnemann, J.$$b1
000034117 7001_ $$0P:(DE-HGF)0$$aKlauser, T.$$b2
000034117 7001_ $$0P:(DE-Juel1)129071$$aWandrey, C.$$b3$$uFZJ
000034117 7001_ $$0P:(DE-Juel1)VDB1625$$aTakors, R.$$b4$$uFZJ
000034117 773__ $$0PERI:(DE-600)2035041-7$$a10.1002/1522-2640(200112)73:12<1615::AID-CITE1615>3.0.CO;2-6$$gVol. 73, p. 1615 - 1621$$p1615 - 1621$$q73<1615 - 1621$$tChemie - Ingenieur - Technik$$v73$$x0009-286X$$y2001
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000034117 9141_ $$y2001
000034117 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000034117 9201_ $$0I:(DE-Juel1)VDB56$$gIBT$$kIBT-2$$lBiotechnologie 2$$x0$$zab 31.10.10 weitergeführt IBG-1
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