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@ARTICLE{Curves:34117,
      author       = {Curves, S. and Linnemann, J. and Klauser, T. and Wandrey,
                      C. and Takors, R.},
      title        = {{R}ecombinant protein production with pichia pastoris in
                      continuous fermentation - kinetic analysis of growth and
                      production formation},
      journal      = {Chemie - Ingenieur - Technik},
      volume       = {73},
      issn         = {0009-286X},
      address      = {Weinheim},
      publisher    = {Wiley-VCH Verl.},
      reportid     = {PreJuSER-34117},
      pages        = {1615 - 1621},
      year         = {2001},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Continuous fermentation was applied to the production of
                      recombinant human chymotrypsinogen B (hCTRB) by the
                      methylotrophic yeast Pichia pastoris as a tool for the
                      kinetic analysis of growth and product formation. Using
                      methanol as the sole source of carbon, energy, and
                      induction, cell growth could be described by a
                      non-competitive M ON approach. The maximum growth rate p a,,
                      was determined to be 0.084 h(-1) and the K-M-value for
                      methanol to 0.22 g L-1, respectively. With respect to
                      product formation a similar model was established exhibiting
                      a methanol concentration of 0.13 g L-1 as the K-M-value and
                      a maximum biomass-specific product-formation rate Of pi(max)
                      = 0.23 mg g(-1) h(-1). The production of hCTRB was strictly
                      growth-coupled.The data provided covers the range of
                      methanol concentrations between 0 and 4 g L-1. Substrate
                      concentrations exceeding this upper value led to a complete
                      collapse of product formation. This change in phenotype
                      turned out to be irreversible indicating a genetic
                      instability of transformed Pichia pastoris caused by excess
                      methanol.},
      keywords     = {J (WoSType)},
      cin          = {IBT-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Verfahrenstechnik zur mikrobiellen Gewinnung von
                      Primärmetaboliten},
      pid          = {G:(DE-Juel1)FUEK93},
      shelfmark    = {Engineering, Chemical},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000173288200018},
      doi          = {10.1002/1522-2640(200112)73:12<1615::AID-CITE1615>3.0.CO;2-6},
      url          = {https://juser.fz-juelich.de/record/34117},
}