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| Hauptseite > Publikationsdatenbank > Recombinant protein production with pichia pastoris in continuous fermentation - kinetic analysis of growth and production formation > print |
| Hauptseite > Publikationsdatenbank > Recombinant protein production with pichia pastoris in continuous fermentation - kinetic analysis of growth and production formation > print |
| 001 | 34117 | ||
| 005 | 20180210141456.0 | ||
| 024 | 7 | _ | |2 DOI |a 10.1002/1522-2640(200112)73:12<1615::AID-CITE1615>3.0.CO;2-6 |
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| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Engineering, Chemical |
| 100 | 1 | _ | |a Curves, S. |0 P:(DE-Juel1)VDB4399 |b 0 |u FZJ |
| 245 | _ | _ | |a Recombinant protein production with pichia pastoris in continuous fermentation - kinetic analysis of growth and production formation |
| 260 | _ | _ | |a Weinheim |b Wiley-VCH Verl. |c 2001 |
| 300 | _ | _ | |a 1615 - 1621 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Chemie Ingenieur Technik |x 0009-286X |0 1215 |y 12 |v 73 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Continuous fermentation was applied to the production of recombinant human chymotrypsinogen B (hCTRB) by the methylotrophic yeast Pichia pastoris as a tool for the kinetic analysis of growth and product formation. Using methanol as the sole source of carbon, energy, and induction, cell growth could be described by a non-competitive M ON approach. The maximum growth rate p a,, was determined to be 0.084 h(-1) and the K-M-value for methanol to 0.22 g L-1, respectively. With respect to product formation a similar model was established exhibiting a methanol concentration of 0.13 g L-1 as the K-M-value and a maximum biomass-specific product-formation rate Of pi(max) = 0.23 mg g(-1) h(-1). The production of hCTRB was strictly growth-coupled.The data provided covers the range of methanol concentrations between 0 and 4 g L-1. Substrate concentrations exceeding this upper value led to a complete collapse of product formation. This change in phenotype turned out to be irreversible indicating a genetic instability of transformed Pichia pastoris caused by excess methanol. |
| 536 | _ | _ | |a Verfahrenstechnik zur mikrobiellen Gewinnung von Primärmetaboliten |c 41.40.0 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK93 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science |
| 650 | _ | 7 | |a J |2 WoSType |
| 700 | 1 | _ | |a Linnemann, J. |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Klauser, T. |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Wandrey, C. |0 P:(DE-Juel1)129071 |b 3 |u FZJ |
| 700 | 1 | _ | |a Takors, R. |0 P:(DE-Juel1)VDB1625 |b 4 |u FZJ |
| 773 | _ | _ | |a 10.1002/1522-2640(200112)73:12<1615::AID-CITE1615>3.0.CO;2-6 |g Vol. 73, p. 1615 - 1621 |p 1615 - 1621 |q 73<1615 - 1621 |0 PERI:(DE-600)2035041-7 |t Chemie - Ingenieur - Technik |v 73 |y 2001 |x 0009-286X |
| 909 | C | O | |o oai:juser.fz-juelich.de:34117 |p VDB |
| 913 | 1 | _ | |k 41.40.0 |v Verfahrenstechnik zur mikrobiellen Gewinnung von Primärmetaboliten |l Biotechnologie |b Lebenswissenschaften |0 G:(DE-Juel1)FUEK93 |x 0 |
| 914 | 1 | _ | |y 2001 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k IBT-2 |l Biotechnologie 2 |g IBT |z ab 31.10.10 weitergeführt IBG-1 |0 I:(DE-Juel1)VDB56 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)3994 |
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| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
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