% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@PHDTHESIS{Jelinek:34345,
      author       = {Jelinek, Nanni},
      title        = {{K}ultivierung hämatopoetischer {Z}ellen : {K}okultur im
                      {F}estbettreaktor},
      volume       = {3863},
      issn         = {0944-2952},
      school       = {Techn. Hochsch. Aachen},
      type         = {Dr. (FH)},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {PreJuSER-34345, Juel-3863},
      series       = {Berichte des Forschungszentrums Jülich},
      pages        = {X, 192 p.},
      year         = {2000},
      note         = {Record converted from VDB: 12.11.2012; Aachen, Techn.
                      Hochsch., Diss.},
      abstract     = {The ex vivo expansion of hematopoietic cells is an
                      important technique for many medical applications. The
                      cultivation concept in this study was the ex vivo simulation
                      of the human bone marrow by co-culture of hematopoietic and
                      stromal cells in a fixed-bed bioreactor . Thereby the cells
                      are immobilized on macroporous collagen microcarriers. A
                      miniaturized loop-reactor was established for screening
                      purposes. For the full supply with primary CD34<sup>+</sup>-
                      cells a cord blood bank was built up. In preliminary
                      investigations two stromal cellines were characterized and
                      the harvest of immobilized cells established by enzymatic
                      lysis of the carriers. The relevant parameters for the
                      co-culture were optimized in the miniaturized loop- reactor.
                      In this system the mononuclear cells (MNC) were expanded up
                      to 454 fold, the colony-forming-cells (CFC) up to 26 fold
                      and the cobblestone-area-forming cells (CAFC) up to 18 fold.
                      In this system the SCm-mouse repopulating cells (SRC) were
                      maintained in contrast to a parallel cultivation in a
                      suspension system. This demonstrates the positive effect of
                      the stromal based, immobilized culture compared to the
                      suspension culture of this cells. Based on the optimization
                      in the screening system the hematopoietic cells were
                      successfully expanded in the fixed-bed bioreactor. The
                      co-culture with the stromal cellline SVSl and the cytokines
                      IL-3, TPO, SCF and FL led to the best results. In this
                      system the MNC were expanded maximal 103 fold, the CFU-GM
                      114 fold and the CAFC 15 fold. These data correspond the
                      medical yardstick demonstrating the clinical relevance of
                      the results presented in this study.},
      cin          = {IBT-2},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Verfahrenstechnik der Gewinnung},
      pid          = {G:(DE-Juel1)FUEK94},
      typ          = {PUB:(DE-HGF)11 / PUB:(DE-HGF)3},
      url          = {https://juser.fz-juelich.de/record/34345},
}