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@PHDTHESIS{Schmidt:34349,
      author       = {Schmidt, Sebastian},
      title        = {{V}erfahrenstechnik für die {K}ultivierung
                      hämatopoetischer {Z}ellen},
      volume       = {3830},
      issn         = {0944-2952},
      school       = {Techn. Hochsch. Aachen},
      type         = {Dr. (FH)},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {PreJuSER-34349, Juel-3830},
      series       = {Berichte des Forschungszentrums Jülich},
      pages        = {XIII, 250 p.},
      year         = {2000},
      note         = {Record converted from VDB: 12.11.2012; Aachen, Techn.
                      Hochsch., Diss.},
      abstract     = {In this work a system of culture techniques was developed
                      for the ex vivo expansion of hematopoietic cells, which is
                      of major importance in cancer therapy. For stroma dependent
                      cocultivation, the fixed bed reactor and the 1 mL
                      miniaturized loop reactor allow the cultivation for clinical
                      and screening purposes, respectively. Here the cells are
                      immobilized on macroporous carriers . For a suspension
                      culture a reactor was developed that has a working volume
                      between 40 and 550 mL. An integrated filtration allows a
                      continuous cultivation up to densities of 1 . 107C/mL.
                      Finally, a small scale spinner vessel of 10 mL suspension
                      offers the possibility of a screening in suspended culture.
                      Additionally, some relevant parameters for the cultivation
                      of hematopoietic progenitor cells were investigated. It was
                      shown, that the materials the cultivation systems are made
                      of have an influence on the cultivation, but in technical
                      systems it is to small to be relevant . An optimal
                      osmolality of the culture was found at 0,31-0,32 Osmol/kg .
                      Besides the progenitor cells, T-lymphocytes were cultivated
                      as an example of a more differentiated cell type . After the
                      successful cultivation in the new suspension reactor system,
                      a standard 20L stirred vessel was used to obtain a
                      clinically relevant final cell number of 2,9 - 101° cells.
                      The new suspension systems was used also in a clinical study
                      phase I/II for cancer therapy of children. Cells obtained
                      from the reactor were transfused to a patient, who showed no
                      negative side effects from the therapy},
      cin          = {IBT-2},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Verfahrenstechnik der Gewinnung},
      pid          = {G:(DE-Juel1)FUEK94},
      typ          = {PUB:(DE-HGF)11 / PUB:(DE-HGF)3},
      url          = {https://juser.fz-juelich.de/record/34349},
}