Hauptseite > Publikationsdatenbank > Verfahrenstechnik für die Kultivierung hämatopoetischer Zellen > print

001     34349
005     20200610184617.0
024 7 _ |2 Handle
|a 2128/20660
037 _ _ |a PreJuSER-34349
088 1 _ |a Juel-3830
088 _ _ |a Juel-3830
|2 JUEL
100 1 _ |0 P:(DE-Juel1)VDB1433
|a Schmidt, Sebastian
|b 0
|e Corresponding author
|u FZJ
245 _ _ |a Verfahrenstechnik für die Kultivierung hämatopoetischer Zellen
260 _ _ |a Jülich
|b Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag
|c 2000
300 _ _ |a XIII, 250 p.
336 7 _ |0 PUB:(DE-HGF)11
|2 PUB:(DE-HGF)
|a Dissertation / PhD Thesis
336 7 _ |0 PUB:(DE-HGF)3
|2 PUB:(DE-HGF)
|a Book
336 7 _ |0 2
|2 EndNote
|a Thesis
336 7 _ |2 DRIVER
|a doctoralThesis
336 7 _ |2 BibTeX
|a PHDTHESIS
336 7 _ |2 DataCite
|a Output Types/Dissertation
336 7 _ |2 ORCID
|a DISSERTATION
490 0 _ |0 PERI:(DE-600)2414853-2
|8 546
|a Berichte des Forschungszentrums Jülich
|v 3830
|x 0944-2952
500 _ _ |a Record converted from VDB: 12.11.2012
502 _ _ |a Aachen, Techn. Hochsch., Diss.
|b Dr. (FH)
|c Techn. Hochsch. Aachen
|d 2000
520 _ _ |a In this work a system of culture techniques was developed for the ex vivo expansion of hematopoietic cells, which is of major importance in cancer therapy. For stroma dependent cocultivation, the fixed bed reactor and the 1 mL miniaturized loop reactor allow the cultivation for clinical and screening purposes, respectively. Here the cells are immobilized on macroporous carriers . For a suspension culture a reactor was developed that has a working volume between 40 and 550 mL. An integrated filtration allows a continuous cultivation up to densities of 1 . 107C/mL. Finally, a small scale spinner vessel of 10 mL suspension offers the possibility of a screening in suspended culture. Additionally, some relevant parameters for the cultivation of hematopoietic progenitor cells were investigated. It was shown, that the materials the cultivation systems are made of have an influence on the cultivation, but in technical systems it is to small to be relevant . An optimal osmolality of the culture was found at 0,31-0,32 Osmol/kg . Besides the progenitor cells, T-lymphocytes were cultivated as an example of a more differentiated cell type . After the successful cultivation in the new suspension reactor system, a standard 20L stirred vessel was used to obtain a clinically relevant final cell number of 2,9 - 101° cells. The new suspension systems was used also in a clinical study phase I/II for cancer therapy of children. Cells obtained from the reactor were transfused to a patient, who showed no negative side effects from the therapy
536 _ _ |0 G:(DE-Juel1)FUEK94
|2 G:(DE-HGF)
|a Verfahrenstechnik der Gewinnung
|c 41.45.1
|x 0
655 _ 7 |a Hochschulschrift
|x Dissertation (FH)
856 4 _ |u https://juser.fz-juelich.de/record/34349/files/J%C3%BCl_3830_Schmidt.pdf
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/34349/files/J%C3%BCl_3830_Schmidt.pdf?subformat=pdfa
|x pdfa
|y OpenAccess
909 C O |o oai:juser.fz-juelich.de:34349
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
913 1 _ |0 G:(DE-Juel1)FUEK94
|b Lebenswissenschaften
|k 41.45.1
|l Biotechnologie
|v Verfahrenstechnik der Gewinnung
|x 0
914 1 _ |y 2001
915 _ _ |0 StatID:(DE-HGF)0510
|2 StatID
|a OpenAccess
920 1 _ |0 I:(DE-Juel1)VDB56
|g IBT
|k IBT-2
|l Biotechnologie 2
|x 0
|z ab 31.10.10 weitergeführt IBG-1
970 _ _ |a VDB:(DE-Juel1)4245
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a phd
980 _ _ |a I:(DE-Juel1)IBG-1-20101118
980 _ _ |a UNRESTRICTED
980 1 _ |a FullTexts
981 _ _ |a I:(DE-Juel1)IBG-1-20101118

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21