TY  - JOUR
AU  - Takors, R.
TI  - Model-based analysis and optimization of an ISPR approach using reactive extraction for pilot scale L-Phenylalanine production
JO  - Biotechnology progress
VL  - 20
SN  - 8756-7938
CY  - Malden, MA
PB  - Wiley
M1  - PreJuSER-34923
SP  - 57 - 64
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - Based on experimental data from fermentation runs, as well as from L-phenylalanine (l-Phe) separation studies, a simple model is presented that describes the total ISPR approach for on-line L-Phe separation. While fermentation process modeling via a macrokinetic model revealed an L-Phe inhibition constant of 20 +/- 1.35 g/L using recombinant E. coli cells, the reactive-extraction process modeling identified the L-Phe cation diffusion in the aqueous donor film and the transport of the lowly soluble carrier/L-Phe complex in the aqueous acceptor film as the most dominant transfer steps. The corresponding mass transfer coefficients were estimated as k(PheD) = 128 x 10(-7) cm/s (extraction) and k(CPheA) = 178 x 10(-5) cm/s (back-extraction). Simulation studies were performed for the total ISPR approach, which gave hints for strategies of further process optimization.
KW  - Algorithms
KW  - Bioreactors: microbiology
KW  - Computer Simulation
KW  - Escherichia coli: growth & development
KW  - Escherichia coli: metabolism
KW  - Fractional Precipitation
KW  - Industrial Microbiology: methods
KW  - Kinetics
KW  - Models, Biological
KW  - Models, Chemical
KW  - Phenylalanine: biosynthesis
KW  - Phenylalanine: chemistry
KW  - Phenylalanine: isolation & purification
KW  - Pilot Projects
KW  - Quality Control
KW  - Ultrafiltration: methods
KW  - Phenylalanine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:14763824
UR  - <Go to ISI:>//WOS:000188861300008
DO  - DOI:10.1021/bp0257473
UR  - https://juser.fz-juelich.de/record/34923
ER  -