TY  - JOUR
AU  - Elmenhorst, D.
AU  - Basheer, R.
AU  - McCarley, R.W.
AU  - Bauer, A.
TI  - Sleep deprivation increases A1 adenosine receptor density in the rat brain.
JO  - Brain research
VL  - 1258
SN  - 0006-8993
CY  - Amsterdam
PB  - Elsevier
M1  - PreJuSER-3639
SP  - 53 - 58
PY  - 2009
N1  - Sabine Wilms, Markus Cremer and Ramkumar Karthikeyan are gratefully acknowledged for excellent technical assistance and Marcus Holschbach for providing Tritium labeled CPFPX.This work was supported by the Department of Veterans Affairs Medical Research Service Award to RB, the National Institute of Mental Health (NIMH39683) to RWM, the Heinrich Hertz Foundation of the Ministry of Science and Technology, North-Rhine Westfalia, Germany to DE and the German Federal Ministry of Education and Research (Brain Imaging Center West, to DE and AB; and Biopharma Initiative-NeuroAllianz, to AB).
AB  - Adenosine, increasing after sleep deprivation and acting via the A(1) adenosine receptor (A(1)AR), is likely a key factor in the homeostatic control of sleep. This study examines the impact of sleep deprivation on A(1)AR density in different parts of the rat brain with [(3)H]CPFPX autoradiography. Binding of [(3)H]CPFPX was significantly increased in parietal cortex (PAR) (7%), thalamus (11%) and caudate-putamen (9%) after 24 h of sleep deprivation compared to a control group with an undisturbed circadian sleep-wake rhythm. Sleep deprivation of 12 h changed receptor density regionally between -5% and +9% (motor cortex (M1), statistically significant) compared to the circadian control group. These results suggest cerebral A(1)ARs are involved in effects of sleep deprivation and the regulation of sleep. The increase of A(1)AR density could serve the purpose of not only maintaining the responsiveness to increased adenosine levels but also amplifying the effect of sleep deprivation and is in line with a sleep-induced homoeostatic reorganization at the synaptic level.
KW  - Adenosine A1 Receptor Antagonists
KW  - Analysis of Variance
KW  - Animals
KW  - Autoradiography
KW  - Brain: metabolism
KW  - Circadian Rhythm
KW  - Male
KW  - Rats
KW  - Rats, Sprague-Dawley
KW  - Receptor, Adenosine A1: metabolism
KW  - Sleep: physiology
KW  - Sleep Deprivation: metabolism
KW  - Tritium
KW  - Xanthines: metabolism
KW  - 8-cyclopenta-3-(3-fluoropropyl)-1-propylxanthine (NLM Chemicals)
KW  - Adenosine A1 Receptor Antagonists (NLM Chemicals)
KW  - Receptor, Adenosine A1 (NLM Chemicals)
KW  - Xanthines (NLM Chemicals)
KW  - Tritium (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19146833
UR  - <Go to ISI:>//WOS:000264536100006
DO  - DOI:10.1016/j.brainres.2008.12.056
UR  - https://juser.fz-juelich.de/record/3639
ER  -