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@ARTICLE{Netzer:36935,
      author       = {Netzer, R. and Peters-Wendisch, P. and Eggeling, L. and
                      Sahm, H.},
      title        = {{C}o-metabolism of a {N}on-{G}rowth {S}ubstrate:
                      {L}-{S}erine {U}tilization by {C}orynebacterium glutamicum},
      journal      = {Applied and environmental microbiology},
      volume       = {70},
      issn         = {0099-2240},
      address      = {Washington, DC [u.a.]},
      publisher    = {Soc.},
      reportid     = {PreJuSER-36935},
      pages        = {7148 - 7155},
      year         = {2004},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Despite its key position in central metabolism, L-serine
                      does not support the growth of Corynebacterium glutamicum.
                      Nevertheless, during growth on glucose, L-serine is consumed
                      at rates up to 19.4 +/- 4.0 nmol min(-1) (mg [dry
                      weight])(-1), resulting in the complete consumption of 100
                      mM L-serine in the presence of 100 mM glucose and an
                      increased growth yield of about $20\%.$ Use of 13C-labeled
                      L-serine and analysis of cellularly derived metabolites by
                      nuclear magnetic resonance spectroscopy revealed that the
                      carbon skeleton of L-serine is mainly converted to
                      pyruvate-derived metabolites such as L-alanine. The sdaA
                      gene was identified in the genome of C. glutamicum, and
                      overexpression of sdaA resulted in (i) functional L-serine
                      dehydratase (L-SerDH) activity, and therefore conversion of
                      L-serine to pyruvate, and (ii) growth of the recombinant
                      strain on L-serine as the single substrate. In contrast,
                      deletion of sdaA decreased the L-serine cometabolism rate
                      with glucose by $47\%$ but still resulted in degradation of
                      L-serine to pyruvate. Cystathionine beta-lyase was
                      additionally found to convert L-serine to pyruvate, and the
                      respective metC gene was induced 2.4-fold under high
                      internal L-serine concentrations. Upon sdaA overexpression,
                      the growth rate on glucose is reduced $36\%$ from that of
                      the wild type, illustrating that even with glucose as a
                      single substrate, intracellular L-serine conversion to
                      pyruvate might occur, although probably the weak affinity of
                      L-SerDH (apparent Km, 11 mM) prevents substantial L-serine
                      degradation.},
      keywords     = {Bacterial Proteins: genetics / Bacterial Proteins:
                      metabolism / Carbon Isotopes: metabolism / Corynebacterium
                      glutamicum: growth $\&$ development / Corynebacterium
                      glutamicum: metabolism / Culture Media / Gene Deletion /
                      Glucose: metabolism / L-Serine Dehydratase: genetics /
                      L-Serine Dehydratase: metabolism / Magnetic Resonance
                      Spectroscopy / Oligonucleotide Array Sequence Analysis /
                      Serine: metabolism / Bacterial Proteins (NLM Chemicals) /
                      Carbon Isotopes (NLM Chemicals) / Culture Media (NLM
                      Chemicals) / Glucose (NLM Chemicals) / Serine (NLM
                      Chemicals) / L-Serine Dehydratase (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBT-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB55},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK256},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:15574911},
      pmc          = {pmc:PMC535176},
      UT           = {WOS:000225719300026},
      doi          = {10.1128/AEM.70.12.7148-7155.2004},
      url          = {https://juser.fz-juelich.de/record/36935},
}