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@ARTICLE{Prichard:37047,
author = {Prichard, J. and Tomos, A. D. and Farrar, J. F. and
Minchin, P. E. H. and Gould, N. and Paul, M. J. and MacRae,
E. A. and Ferrieri, R. A. and Gray, D. W. and Thorpe, M. R.},
title = {{T}urgor, solute import and growth in maize roots treated
with galactose},
journal = {Functional plant biology},
volume = {31},
issn = {1445-4408},
address = {Collingwood, Victoria},
publisher = {CSIRO Publ.},
reportid = {PreJuSER-37047},
pages = {1095 - 1103},
year = {2004},
note = {Record converted from VDB: 12.11.2012},
abstract = {It has been observed that extension growth in maize roots
is almost stopped by exposure to 5 mM D-galactose in the
root medium, while the import of recent photoassimilate into
the entire root system is temporarily promoted by the same
treatment. The aim of this study was to reconcile these two
apparently incompatible observations. We examined events
near the root tip before and after galactose treatment since
the tip region is the site of elongation and of high carbon
deposition in the root. The treatment rapidly decreased root
extension along the whole growing zone. In contrast, turgor
pressure, measured directly with the pressure probe in the
cortical cells of the growing zone, rapidly increased by
0.15 MPa within the first hour following treatment, and the
increase was maintained over the following 24 h. Both
tensiometric measurements and a comparison of turgor
pressure with local growth rate demonstrated that a rapid
tightening of the cell wall caused the reduction in growth.
Single cell sampling showed cell osmotic pressure increased
by 0.3 MPa owing to accumulation of both organic and
inorganic solutes. The corresponding change in cell water
potential was a rise from -0.18 MPa to approximately zero.
More mature cells at 14 mm from the root tip (just outside
the growing region) showed a qualitatively similar
response.Galactose treatment rapidly increased the import of
recently fixed carbon (RFC) into the whole root as deduced
by C-11 labelling of photoassimilate. In contrast, there was
a significant decrease in import of recently fixed carbon
into the apical 5 mm concomitant with the increase in turgor
in this region. No decrease in import of recently fixed
carbon was observed 5-15 mm from the root tip despite the
increase in cortical cell turgor. These data are consistent
with direct symplastic connections between the growing cells
and the phloem supplying the solutes in the apical, but not
the basal, regions of the growing zone. Hence, the
inhibition of growth and the elevation of solute import
induced by galactose are spatially separated within the
root.},
keywords = {J (WoSType)},
cin = {ICG-III},
ddc = {580},
cid = {I:(DE-Juel1)VDB49},
pnm = {Chemie und Dynamik der Geo-Biosphäre},
pid = {G:(DE-Juel1)FUEK257},
shelfmark = {Plant Sciences},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000225301200005},
doi = {10.1071/FP04082},
url = {https://juser.fz-juelich.de/record/37047},
}