%0 Journal Article
%A Nyquist, R. M.
%A Ataka, K.
%A Heberle, J.
%T The molecular mechanism of membrane proteins probed by evanescent infrared waves
%J ChemBioChem
%V 5
%@ 1439-4227
%C Weinheim
%I Wiley-VCH
%M PreJuSER-38377
%P 431 - 436
%D 2004
%Z Record converted from VDB: 12.11.2012
%X The catalytic action of membrane proteins is vital to many cellular processes. Yet the molecular mechanisms remain poorly understood. We describe here the technique of evanescent infrared difference spectroscopy as a tool to decipher the structural changes associated with the enzymatic action of membrane proteins. Functional changes as minute as the protonation state of individual amino acid side chains can be observed and linked to interactions with a ligand, agonist, effector, or redox partner.
%K Bacteriorhodopsins: chemistry
%K Bacteriorhodopsins: metabolism
%K Electron Transport Complex IV: chemistry
%K Electron Transport Complex IV: metabolism
%K Membrane Proteins: chemistry
%K Membrane Proteins: metabolism
%K Rhodopsin: chemistry
%K Rhodopsin: metabolism
%K Spectrophotometry, Infrared: methods
%K Membrane Proteins (NLM Chemicals)
%K Bacteriorhodopsins (NLM Chemicals)
%K Rhodopsin (NLM Chemicals)
%K Electron Transport Complex IV (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:15185365
%U <Go to ISI:>//WOS:000220773500004
%R 10.1002/cbic.200300687
%U https://juser.fz-juelich.de/record/38377