TY  - JOUR
AU  - Bednarz, T.
AU  - Losi, A.
AU  - Gärtner, W.
AU  - Hegemann, P.
AU  - Heberle, J.
TI  - Functional variations among LOV domains as revealed by FTIR difference spectroscopy
JO  - Photochemical & photobiological sciences
VL  - 3
SN  - 1474-905X
CY  - Cambridge
PB  - Royal Society of Chemistry
M1  - PreJuSER-38378
SP  - 575 - 579
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - The two LOV domains, LOV1 and LOV2, from Chlamydomonas reinhardtii were investigated by light-induced FT-IR difference spectroscopy and compared to the LOV domain of Bacillus subtilis (YtvA-LOV). It is shown that the two S-H conformations of the reactive LOV1 cysteine C57(1) are exposed to environments of different hydrogen bonding strength. Thus, the two rotamer configurations of C57 might be related to the fact that the triplet state decays bi-exponentially into the LOV1-390 photoproduct. Exchange of the two other cysteines of LOV1 (C32S and C83S) does not alter the S-H stretching band providing evidence that this band feature arises solely from C57. The reactive cysteine of LOV2 from Chlamydomonas reinhardtii (C250) and of YtvA-LOV (C62) exhibit both a homogenous S-H stretching vibrational band which suggests a single conformer of the amino acid side chain. Finally, the FT-IR difference spectrum of YtvA from Bacillus subtilis comprising the light absorbing LOV domain and the putative signaling STAS (sulfate transporter/antisigma-factor antagonist) domain, reveals conformational changes in the latter after blue-light excitation.
KW  - Animals
KW  - Bacillus subtilis: physiology
KW  - Bacillus subtilis: radiation effects
KW  - Bacterial Proteins: chemistry
KW  - Bacterial Proteins: genetics
KW  - Bacterial Proteins: physiology
KW  - Chlamydomonas reinhardtii: physiology
KW  - Chlamydomonas reinhardtii: radiation effects
KW  - Crystallography, X-Ray
KW  - Protein Conformation
KW  - Spectrometry, Fluorescence
KW  - Spectroscopy, Fourier Transform Infrared: methods
KW  - Bacterial Proteins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15170487
UR  - <Go to ISI:>//WOS:000221746800012
DO  - DOI:10.1039/b400976b
UR  - https://juser.fz-juelich.de/record/38378
ER  -